@article {WANG2025128234,
	title = {High-throughput direct profiling of natural products in plant leaves using ambient ionization high-resolution mass spectrometry},
	journal = {Talanta},
	year = {2025},
	pages = {128234},
	abstract = {<p>Plant leaves contain natural products that may have nutritional and medicinal values. In this work, a modified ambient ion source of angled direct analysis in real time (aDART) and high-resolution mass spectrometry (HRMS) was used for direct surface analysis of different plant leaves. Untreated leaves, leaves with the wax layer removed, and leaf imprints were all successfully analyzed by aDART-HRMS in min, observing abundant natural product information including esters, amino acids, saccharides, flavonoids, alkaloids, alcohols, and ketones/aldehydes. Different plant samples have their unique features. Fresh leaves facilitate instant analysis without sample preparation, dewaxed leaves can help expose chemicals concealed by the epidermis, and the leaf imprints can better reveal substances inside the leaf tissues. Traditional leaf extracts were compared and analyzed using DART-HRMS, and it was shown that more compounds were discovered by surface analysis. aDART-HRMS is capable of obtaining samples\&rsquo; composition directly from plant leaves, without destroying leaf morphology and tedious sample preparation. This modified aDART-MS technology provides a simple and robust ambient ionization method for direct characterization of natural products contained in various plant samples.</p>},
	keywords = {angled direct analysis in real time, High-resolution mass spectrometry, natural products, plant leaves, surface analysis},
	issn = {0039-9140},
	doi = {https://doi.org/10.1016/j.talanta.2025.128234},
	url = {https://www.sciencedirect.com/science/article/pii/S0039914025007246},
	author = {Saiting Wang and Tiange Gu and Hongli Li and Xuefeng Jiang and David D. Y. Chen}
}
@article {BAI2024139802,
	title = {Ambient mass spectrometry imaging of food natural products by angled direct analysis in real time high-resolution mass spectrometry},
	journal = {Food Chemistry},
	volume = {454},
	year = {2024},
	month = {05/2024},
	pages = {139802},
	type = {Research},
	abstract = {<p>Direct surface analysis in ambient conditions provides information on the position and chemical composition of an object at the time of investigation. An angled sampling probe is developed in this work for direct analysis in real time (DART) ionization high-resolution mass spectrometry. The DART ion source and the interface were modified for improved surface resolution, increased ion transfer efficiency, as well as enabling two-dimensional surface scanning. The angled probe DART-MS system was used for investigating a variety of food samples including fruit peels, ginseng root, plant leaves and sections of radish. Abundant signals and distinct chemical profiles are obtained in seconds, and spatial distribution of different molecules across the sample surfaces can be observed. In addition, the developed system can quickly identify the chemical changes when the surfaces were treated. The method is capable of directly evaluating food sample surfaces with different shapes, hardness, and conditions, without any sample pretreatments.</p>},
	keywords = {Ambient condition surface analysis, Angled sampling, Direct analysis in real time ionization, High-resolution mass spectrometry, Molecular distribution},
	issn = {0308-8146},
	doi = {https://doi.org/10.1016/j.foodchem.2024.139802},
	url = {https://www.sciencedirect.com/science/article/pii/S0308814624014523},
	author = {Zhiru Bai and Zhengyan Zhao and Saiting Wang and Hongli Li and David D. Y. Chen}
}
