@article {1905,
	title = {Optimal attachment position and linker length promote native-like character of cavitand-based template-assembled synthetic proteins (TASPs)},
	journal = {Chemistry-a European Journal},
	volume = {13},
	number = {13},
	year = {2007},
	note = {Seo, Emily S. Scott, Walter R. P. Straus, Suzana K. Sherman, John C.},
	pages = {3596-3605},
	abstract = {We have designed, synthesised and characterised a series of template-assembled de novo four-helix bundles, each differing in the linker length between the template and the peptides. The helix is based on an earlier peptide sequence: EELLKKLEELLKKLG (first-generation sequence), which was designed to link the hydrophilic/hydrophobic interface of the helices. Increasing or decreasing the linker length by one glycine residue had a significant effect on the structure and properties of the template-assembled synthetic proteins (TASPs). Here, the effect of the linker length is further probed by linking the peptides closer to the hydrophobic face by using the second-generation sequence, AEELLKKLEELLKKG, in an effort to improve the packing between the helices and to better understand the helical bundles. The peptides were synthesised with 0-4 Gly linker residues and linked onto a cavitand template. The proteins were found to be alpha-helical, stable to guanidine hydrochloride (GuHCl) and to unfold cooperatively. However, their stabilities toward GuHCl, propensity to self-aggregate and structural specificity differed. The two-glycine variant of the second-generation series demonstrated the highest stability and most native-like character of all the mononeric TASPs in both the first- and second-generation series. The structural specificity of this two glycine variant is comparable to that of other known native-like de novo proteins. Molecular dynamics simulations showed that the two-glycine variant contains helices that are tilted with respect to the cavitand template and may account for its unique properties.},
	isbn = {0947-6539},
	url = {<Go to ISI>://000246434300002},
	author = {Seo, E. S. and Scott, W. R. P. and Straus, S. K. and Sherman, J. C.}
}
