@article {4301,
	title = {Calcium binding by the N-terminal cellulose-binding domain from Cellulomonas fimi beta-1,4-glucanase CenC},
	journal = {Biochemistry},
	volume = {37},
	number = {37},
	year = {1998},
	note = {ISI Document Delivery No.: 125NRTimes Cited: 16Cited Reference Count: 33},
	month = {Sep},
	pages = {12772-12781},
	type = {Article},
	abstract = {The interaction of the N-terminal cellulose-binding domain, CBDN1, from Cellulomonas fimi beta-1,4-glucanase CenC with calcium was investigated using NMR spectroscopy and calorimetry. CBDN1 binds a single calcium ion with an equilibrium association constant of approximately 10(5) M-1 at 35 degrees C and pH 6.0. Binding is exothermic (-42 +/- 2 kJ mol(-1)) under these conditions and is accompanied by a small negative change in heat capacity (Delta C-p = -0.41 +/- 0.16 kJ mol(-1) K-1). From an NMR line shape analysis, the rate constants for calcium association and dissociation were found to be (5 +/- 7) x 10(7) s(-1) M-1 and (4.5 +/- 0.6) x 10(2) s(-1), respectively. The rapid association kinetics indicate that the calcium-binding site on CBDN1 is accessible and, to the first approximation, preformed. Based on patterns of chemical shift perturbations, and structural comparisons with the Bacillus sp. 1,3-1,4-beta-glucanases, the backbone carbonyl oxygens of Thr8, Gly30, and Asp 142 and a side chain carboxyl oxygen of Asp 142 are postulated to form the calcium-binding site of CBDN1. Consistent with the calcium-independent affinity of CBDN1 for cellopentaose, this exposed site is located on the face of CBDN1 opposite to that forming the oligosaccharide-binding cleft. The midpoint denaturation temperature of CBDN1 is increased by approximately 8 degrees C at pH 6.0 in the presence of saturating amounts of calcium, confirming that metal ion binding is thermodynamically linked to native-state stability.},
	keywords = {4-GLUCANOHYDROLASE, AFFINITY, CA2+, CRYSTAL-STRUCTURE, GLUCANASES, NUCLEAR-MAGNETIC-RESONANCE, RESOLUTION, SPECTROSCOPY, THERMODYNAMICS, THERMOSTABILITY},
	isbn = {0006-2960},
	url = {<Go to ISI>://000076244100011},
	author = {Johnson, P. E. and Creagh, A. L. and Brun, E. and Joe, K. and Tomme, P. and Haynes, C. A. and McIntosh, L. P.}
}
