@article {1532,
	title = {An ent-kaurene that inhibits mitotic chromosome movement and binds the kinetochore protein Ran-binding protein 2},
	journal = {Acs Chemical Biology},
	volume = {1},
	number = {7},
	year = {2006},
	note = {Rundle, Natalie T. Nelson, Jim Flory, Mark R. Joseph, Jomon Th{\textquoteright}ng, John Aebersold, Ruedi Dasso, Mary Andersen, Raymond J. Roberge, Michel},
	pages = {443-450},
	abstract = {Using a chemical genetics screen, we have identified ent-15-oxokaurenoic acid (EKA) as a chemical that causes prolonged mitotic arrest at a stage resembling prometaphase. EKA inhibits the association of the mitotic motor protein centromeric protein E with kinetochores and inhibits chromosome movement. Unlike most antimitotic agents, EKA does not inhibit the polymerization or depolymerization of tubulin. To identify EKA-interacting proteins, we used a cell-permeable biotinylated form that retains biological activity to isolate binding proteins from living cells. Mass spectrometric analysis identified six EKA-binding proteins, including Ran-binding protein 2, a kinetochore protein whose depletion by small interfering RNA causes a similar mitotic arrest phenotype.},
	isbn = {1554-8929},
	url = {<Go to ISI>://000243894000018},
	author = {Rundle, N. T. and Nelson, J. and Flory, M. R. and Joseph, J. and Th{\textquoteright}ng, J. and Aebersold, R. and Dasso, M. and Andersen, R. J. and Roberge, M.}
}
