@article {MacLennan201879,
	title = {Capillary electrophoresis-mass spectrometry for targeted and untargeted analysis of the sub-5 kDa urine metabolome of patients with prostate or bladder cancer: A feasibility study},
	journal = {Journal of Chromatography B},
	volume = {1074{\textendash}1075},
	year = {2018},
	pages = {79 - 85},
	abstract = {Abstract Targeted and untargeted analyses of the sub-5 kDa urine metabolome of genitourinary cancer patients (prostate and/or bladder) were performed without chemical derivatization using capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS). For targeted analysis, endogenous levels of sarcosine and 5 other amino acid metabolites implicated in the progression of prostate cancer were quantified in four patients and in a pooled urine sample from healthy volunteers. An untargeted analysis (m/z 50 to 850) of patient urine was performed using the same CE-ESI-MS system identifying over 400 distinct molecular features per patient. All patient urine samples were collected at prostatectomy/cystectomy via catheter. Patient urine samples were filtered by centrifugation, with endogenous sarcosine enriched by solid-phase extraction, and the processed samples loaded onto CE-ESI-MS for analysis. Diagnostic information, digital pathological slides, and tissue samples were collected and stored in a comprehensive biobanking database. The introduction of urine sample collection into the surgery workflow was facile and is a promising strategy for addressing the translational research challenge of moving smoothly from {\textquotedblleft}chromatogram to nomogram{\textquotedblright}.},
	keywords = {amino acids},
	issn = {1570-0232},
	doi = {https://doi.org/10.1016/j.jchromb.2018.01.007},
	url = {https://www.sciencedirect.com/science/article/pii/S1570023217317051},
	author = {Matthew S. MacLennan and Miranda G.M. Kok and Laiel Soliman and Alan So and Antonio Hurtado-Coll and David D.Y. Chen}
}
