@article { ISI:000370878000004,
	title = {Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-beta-glucosidases},
	journal = {FEBS LETTERS},
	volume = {590},
	number = {4},
	year = {2016},
	month = {FEB},
	pages = {461-468},
	abstract = {Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-beta-glucosidase from CAZy family GH1. We further present the three-dimensional structure of the inactivated enzyme, which reveals the constellation of active site residues responsible for the enzyme{\textquoteright}s specificity and confirms the covalent nature of the inactivation.},
	issn = {0014-5793},
	doi = {10.1002/1873-3468.12059},
	author = {Kwan, David H. and Jin, Yi and Jiang, Jianbing and Chen, Hong-Ming and Koetzler, Miriam P. and Overkleeft, Herman S. and Davies, Gideon J. and Withers, Stephen G.}
}
