@article { ISI:000368577700005,
	title = {Synthesis and evaluation of a series of 6-chloro-4-methylumbelliferyl glycosides as fluorogenic reagents for screening metagenomic libraries for glycosidase activity},
	journal = {CARBOHYDRATE RESEARCH},
	volume = {421},
	year = {2016},
	month = {FEB 8},
	pages = {33-39},
	abstract = {Screening of large enzyme libraries such as those derived from metagenomic sources requires sensitive substrates. Fluorogenic glycosides typically offer the best sensitivity but typically must be used in a stopped format to generate good signal. Use of fluorescent phenols of pKa < 7, such as halogenated coumarins, allows direct screening at neutral pH. The synthesis and characterisation of a set of nine different glycosides of 6-chloro-4-methylumbelliferone are described. The use of these substrates in a pooled format for screening of expressed metagenomic libraries yielded a {\textquoteleft}{\textquoteleft}hit rate{{\textquoteright}{\textquoteright}} of 1 in 60. Hits were then readily deconvoluted with the individual substrates in a single plate to identify specific activities within each clone. The use of such a collection of substrates greatly accelerates the screening process. (C) 2015 Elsevier Ltd. All rights reserved.},
	issn = {0008-6215},
	doi = {10.1016/j.carres.2015.12.010},
	author = {Chen, Hong-Ming and Armstrong, Zachary and Hallam, Steven J. and Withers, Stephen G.}
}
