@article {doi:10.1021/co3001378,
	title = {Toward the Combinatorial Selection of Chemically Modified DNAzyme RNase A Mimics Active Against all-RNA Substrates},
	journal = {ACS Combinatorial Science},
	volume = {15},
	number = {4},
	year = {2013},
	note = {PMID: 23485334},
	pages = {174-182},
	abstract = {The convenient use of SELEX and related combinatorial methods of in vitro selection provides a formidable gateway for the generation of DNA enzymes, especially in the context of improving their potential as gene therapeutic agents. Here, we report on the selection of DNAzyme 12{\textendash}91, a modified nucleic acid catalyst adorned with imidazole, ammonium, and guanidinium groups that provide for efficient M2+-independent cleavage of an all-RNA target sequence (kobs = 0.06 min{\textendash}1). While Dz12{\textendash}91 was selected for intramolecular cleavage of an all-RNA target, it surprisingly cleaves a target containing a lone ribocytosine unit with even greater efficiency (kobs = 0.27 min{\textendash}1) than Dz9{\textendash}86 (kobs = 0.13 min{\textendash}1). The sequence composition of Dz12{\textendash}91 bears a marked resemblance to that of Dz9{\textendash}86 (kobs = 0.0014 min{\textendash}1 with an all-RNA substrate) that was selected from the same library to cleave a target containing a single ribonucleotide. However, small alterations in the sequence composition have a profound impact on the substrate preference and catalytic properties. Indeed, Dz12{\textendash}91 displays the highest known rate enhancement for the M2+-independent cleavage of all-RNA targets. Hence, Dz12{\textendash}91 represents a step toward the generation of potentially therapeutically active DNAzymes and further underscores the usefulness of modified triphosphates in selection experiments.},
	doi = {10.1021/co3001378},
	url = {http://dx.doi.org/10.1021/co3001378},
	author = {Marcel Hollenstein and Christopher J. Hipolito and Curtis H. Lam and David M. Perrin}
}
