@article { ISI:000176173700021,
	title = {Solid-phase oligosaccharide and glycopeptide synthesis using glycosynthases},
	journal = {JOURNAL OF ORGANIC CHEMISTRY},
	volume = {67},
	number = {12},
	year = {2002},
	month = {JUN 14},
	pages = {4143-4149},
	abstract = {Enzymatic approaches for the preparation of oligosaccharides are interesting alternatives to traditional chemical synthesis, the main advantage being the regio- and stereoselectivity offered without the need for protecting groups. The use of solid-phase techniques offers easy workup procedures and the prospect of automatability. Here, we report the first application of glycosynthases to solid-phase oligosaccharide synthesis by use of the 51 kDa serine and glycine mutants of Agrobacterium sp. beta-glucosidase, Abg E358S and E358G. Acceptors were linked to PEGA resin through a backbone amide linker (BAL), and using these mutated enzymes, a galactose moiety was transferred from a donor sugar, U-D-galactosyl fluoride, with high efficiency (>90\%) together with excellent recovery of material. Furthermore, it was demonstrated that a resin-bound model glycopeptide was also an acceptor for the glycosynthase.},
	issn = {0022-3263},
	doi = {10.1021/jo0163445},
	author = {Tolborg, JF and Petersen, L and Jensen, KJ and Mayer, C and Jakeman, DL and WARREN, RAJ and Withers, SG}
}
