@article { ISI:000225738200020,
	title = {Chemo-enzymatic synthesis of 4-methylumbelliferyl beta-(1 -> 4)-D-xylooligosides: new substrates for beta-D-xylanase assays},
	journal = {ORGANIC \& BIOMOLECULAR CHEMISTRY},
	volume = {3},
	number = {1},
	year = {2005},
	pages = {146-151},
	type = {Article},
	abstract = {Transglycosylation catalyzed by a beta-D-xylosidase from Aspergillus sp. was used to synthesize a set of 4-methylumbelliferyl (MU) beta-1{\textendash}>4-D-xylooligosides having the common structure {[}beta-D-Xyl-(1{\textendash}>4)](2-5)-beta- D-Xyl-MU. MU xylobioside synthesized chemically by the condensation of protected MU beta-D-xylopyranoside with ethyl 2,3,4-tri-O-acetyl-1-thio-beta-D-xylopyranoside was used as a substrate for transglycosylation with the beta-D- xylosidase from Aspergillus sp. to produce higher MU xylooligosides. The structures of oligosaccharides obtained were established by H-1 and C-13 NMR spectroscopy and electrospray tandem mass spectrometry. MU beta-D-xylooligosides synthesized were tested as fluorogenic substrates for the GH-10 family beta-D-xylanase from Aspergillus orizae and the GH-11 family beta-D- xylanase I from Trichoderma reesei. Both xylanases released the aglycone from MU xylobioside and the corresponding trioside. With substrates having d.p. 4 and 5, the enzymes manifested endolytic activities, splitting off MU, MUX, and MUX2 primarily.},
	issn = {1477-0520},
	doi = {10.1039/b409583a},
	author = {Eneyskaya, EV and Ivanen, DR and Shabalin, KA and Kulminskaya, AA and Backinowsky, LV and Brumer, H and Neustroev, KN}
}
