@article { ISI:000304055100015,
	title = {A general route to xyloglucan-peptide conjugates for the activation of cellulose surfaces},
	journal = {CARBOHYDRATE RESEARCH},
	volume = {354},
	year = {2012},
	month = {JUN 1},
	pages = {116-120},
	type = {Article},
	abstract = {Cellulose is an attractive supporting matrix for diverse biotechnological applications, including chromatography, diagnostics, and tissue replacement/scaffolding, due to its renewable resource status, low cost, and low non-specific interaction with biomolecules. In an effort to expand the biofunctionality of cellulose materials, we present here a versatile method for the synthesis of xyloglucan-peptide conjugates that harness the strong xyloglucan-cellulose binding interaction for gentle surface modification. Xylogluco-oligosaccharide aminoalditols (XGO-NH2) were coupled to both linear and cyclic peptides, which contained the endothelial cell epitope Arg-Gly-Asp, in a facile two-step approach employing diethyl squarate cross-linking. Subsequent xyloglucan endo-transglycosylase-mediated coupling of the resulting XGO-GRGDS (Gly-Arg-Gly-Asp-Ser) and XGO-c{[}RGDfK]-PEG-PEG (cyclo{[}Arg-Gly-Asp-(D-Phe)-Lys]-PEG-PEG; where PEG is 8-amino-3,6-dioxaoctanoic acid) conjugates into high molecular mass xyloglucan yielded xyloglucan-RGD peptide conjugates suitable for cellulose surface activation. Notably, use of XGO-squaramate as a readily accessible, versatile intermediate overcomes previous limitations of solid-phase synthetic approaches to XGO-peptide conjugates, and furthermore allows the method to be generalized to a wide variety of polypeptides and proteins, as well as diverse primary amino compounds. (C) 2012 Elsevier Ltd. All rights reserved.},
	issn = {0008-6215},
	doi = {10.1016/j.carres.2012.03.038},
	author = {Araujo, Ana Catarina and Nakhai, Azadeh and Ruda, Marcus and Slattegard, Rikard and Gatenholm, Paul and Brumer, Harry}
}
