Our Department

Stephen Withers

Profile

Research and Teaching Interests

Our interests focus primarily upon enzymes that catalyse glycoside formation and hydrolysis, since these play crucial roles in all areas of biology. Applications of our research (see below) range from the development of new catalysts for industrial processes to the design, synthesis and testing of new therapeutics.

Understanding: Mechanistic studies are the foundation of our program and range from the discovery and analysis of "new" mechanisms by which enzymes catalyse these reactions through to development of a deeper understanding of how the enzymes for which we know the fundamental mechanisms achieve such enormous (107 fold) rate accelerations. The approaches adopted involve a combination of synthetic organic chemistry (synthesis of substrate analogues, inhibitors, etc.), molecular biology (cloning, mutagenesis, etc.,) and biochemistry (enzyme purification, kinetics, labeling, peptide mapping). These are often coupled with collaborative crystallographic and NMR studies of protein/ligand complexes to gain insights into structures, dynamics and electrostatics of the species along the enzyme’s reaction coordinates. We also utilise mass spectrometry for peptide mapping and gas phase studies. Central to our mechanistic studies are the databases CAZY and CAZYPEDIA.

Inhibitors of glycosidases have value both as mechanistic probes and as potential diagnostics and therapeutics. Mechanism-based covalent inhibitors, often based upon fluorosugars, have been designed and used by our lab to prove covalent catalysis, to identify active site residues andto visualize structures of reaction intermediates. These, along with non-covalent inhibitors, are being developed as potential therapeutics in conjunction with B.C.’s Centre for Drug Research and Development (CDRD). Trials are currently underway for new treatments for influenza (neuraminidase inhibition), diabetes (α-amylase inhibition) and lysosomal storage diseases (pharmacological chaperones). These candidates were derived both by design / synthesis and by high-throughput screening of natural product extracts.

Engineering: a particular challenge in glycoscience is the efficient assembly of oligosaccharides and glycoproteins – especially on the large scale. We have generated glycosynthases and glycoligases (engineered glycosidases) for the high-yielding assembly of glycosides. Through application of directed evolution approaches that mimic the process of natural selection, but in the laboratory, we are generating improved versions of these enzymes with activities up to 5000 times higher than their parents. Our involvement in the Centre for High-Throughput Biology (CHiBi) is central to such studies. Students interested in genome-wide and high-throughput chemical biology studies are also alerted to our new Genome Sciences and Technology (GSAT) graduate program.

 

Steve Withers Group

Contact

Email: 
Office Room Number(s): 
Chem A333
Office Phone Number: 
604-822-3402
Fax Number: 
604-822-8869

Curriculum Vitae

B.Sc., Bristol (1974); Ph.D., Bristol (M.L. Sinnott, 1977); Postdoctoral, Alberta (N.B. Madsen and B.D. Sykes, 1978-82); Fellow of the Chemical Institute of Canada; Merck, Sharpe and Dohme Award of the CIC (1989); Corday Morgan Medal of the Royal Society of Chemistry (1990); Rutherford Medal, Royal Society of Canada (1992), Khorana Chair of Biological Chemistry, UBC (1997), Hoffman LaRoche Award of the Canadian Society for Chemistry, 1998, Charmian Medal of the Royal Society of Chemistry, U.K., 2001, Whistler Award, International Carbohydrate Organisation, 2002. Scientific Director of the Protein Engineering Network of Centres of Excellence (PENCE)2000-05; Jacob Biely Faculty Research Prize (UBC) 2005; Finalist, NSERC Herzberg Award 2010; R.U. Lemieux Award for Research in Organic Chemistry (CSC) 2011; Director, Centre for High-Throughput Biology (CHiBi) 2008 - present; Canada Research Chair in Chemical Biology, 2008 - present; Fellow of the Royal Society of London, 2012.

Publications

2014

Jongkees, S. A. K. ; Yoo, H. ; Withers, S. G. Mechanistic Insights From Substrate Preference In Unsaturated Glucuronyl Hydrolase. CHEMBIOCHEM 2014, 15, 124-134.
Yu, C. - C. ; Hill, T. ; Kwan, D. H. ; Chen, H. - M. ; Lin, C. - C. ; Wakarchuk, W. ; Withers, S. G. A Plate-Based High-Throughput Activity Assay For Polysialyltransferase From Neisseria Meningitidis. ANALYTICAL BIOCHEMISTRY 2014, 444, 67-74.
Jongkees, S. A. K. ; Withers, S. G. Unusual Enzymatic Glycoside Cleavage Mechanisms. ACCOUNTS OF CHEMICAL RESEARCH 2014, 47, 226-235.
Knott, B. C. ; Momeni, M. Haddad; Crowley, M. F. ; Mackenzie, L. F. ; Goetz, A. W. ; Sandgren, M. ; Withers, S. G. ; Stahlberg, J. ; Beckham, G. T. The Mechanism Of Cellulose Hydrolysis By A Two-Step, Retaining Cellobiohydrolase Elucidated By Structural And Transition Path Sampling Studies. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2014, 136, 321-329.

2013

Bie, H. ; Yin, J. ; He, X. ; Kermode, A. R. ; Goddard-Borger, E. D. ; Withers, S. G. ; James, M. N. G. Insights Into Mucopolysaccharidosis I From The Structure And Action Of Alpha-L-Iduronidase. NATURE CHEMICAL BIOLOGY 2013, 9, 739+.
Armstrong, Z. ; Withers, S. G. Synthesis Of Glycans And Glycopolymers Through Engineered Enzymes. BIOPOLYMERS 2013, 99, 666-674.
Mewis, K. ; Armstrong, Z. ; Song, Y. C. ; Baldwin, S. A. ; Withers, S. G. ; Hallam, S. J. Biomining Active Cellulases From A Mining Bioremediation System. JOURNAL OF BIOTECHNOLOGY 2013, 167, 462-471.
Caner, S. ; Nguyen, N. ; Aguda, A. ; Zhang, R. ; Pan, Y. T. ; Withers, S. G. ; Brayer, G. D. The Structure Of The Mycobacterium Smegmatis Trehalose Synthase Reveals An Unusual Active Site Configuration And Acarbose-Binding Mode(Dagger). GLYCOBIOLOGY 2013, 23, 1075-1083.
F. Shaikh, A. ; van Bueren, A. Lammerts; Davies, G. J. ; Withers, S. G. Identifying The Catalytic Acid/base In Gh29 Alpha-L-Fucosidase Subfamilies. BIOCHEMISTRY 2013, 52, 5857-5864.
Ratananikom, K. ; Choengpanya, K. ; Tongtubtim, N. ; Charoenrat, T. ; Withers, S. G. ; Kongsaeree, P. T. Mutational Analysis In The Glycone Binding Pocket Of Dalbergia Cochinchinensis Beta-Glucosidase To Increase Catalytic Efficiency Toward Mannosides. CARBOHYDRATE RESEARCH 2013, 373, 35-41.
Ludwiczek, M. L. ; D'Angelo, I. ; Yalloway, G. N. ; Brockerman, J. A. ; Okon, M. ; Nielsen, J. E. ; Strynadka, N. C. J. ; Withers, S. G. ; McIntosh, L. P. Strategies For Modulating The Ph-Dependent Activity Of A Family 11 Glycoside Hydrolase. BIOCHEMISTRY 2013, 52, 3138-3156.
Rakic, B. ; Rao, F. V. ; Freimann, K. ; Wakarchuk, W. ; Strynadka, N. C. J. ; Withers, S. G. Structure-Based Mutagenic Analysis Of Mechanism And Substrate Specificity In Mammalian Glycosyltransferases: Porcine St3Gal-I. GLYCOBIOLOGY 2013, 23, 536-545.
Kim, J. - H. ; Resende, R. ; Wennekes, T. ; Chen, H. - M. ; Bance, N. ; Buchini, S. ; Watts, A. G. ; Pilling, P. ; Streltsov, V. A. ; Petric, M. ; Liggins, R. ; Barrett, S. ; McKimm-Breschkin, J. L. ; Niikura, M. ; Withers, S. G. Mechanism-Based Covalent Neuraminidase Inhibitors With Broad-Spectrum Influenza Antiviral Activity. SCIENCE 2013, 340, 71-75.
Thompson, J. ; Pikis, A. ; Rich, J. ; Hall, B. G. ; Withers, S. G. Alpha-Galacturonidase(S): A New Class Of Family 4 Glycoside Hydrolases With Strict Specificity And A Unique Chev Active Site Motif. FEBS LETTERS 2013, 587, 799-803.
Stocker, B. L. ; Jongkees, S. A. K. ; Win-Mason, A. L. ; Dangerfield, E. M. ; Withers, S. G. ; Timmer, M. S. M. The `mirror-Image' Postulate As A Guide To The Selection And Evaluation Of Pyrrolidines As Alpha-L-Fucosidase Inhibitors. CARBOHYDRATE RESEARCH 2013, 367, 29-32.
Chan, P. H. W. ; Cheung, A. H. ; Okon, M. ; Chen, H. - M. ; Withers, S. G. ; McIntosh, L. P. Investigating The Structural Dynamics Of Alpha-1,4-Galactosyltransferase C From Neisseria Meningitidis By Nuclear Magnetic Resonance Spectroscopy. BIOCHEMISTRY 2013, 52, 320-332.
Yu, C. - C. ; De Huang, L. -; Kwan, D. H. ; Wakarchuk, W. W. ; Withers, S. G. ; Lin, C. - C. A Glyco-Gold Nanoparticle Based Assay For Alpha-2,8-Polysialyltransferase From Neisseria Meningitidis. CHEMICAL COMMUNICATIONS 2013, 49, 10166-10168.

2012

Vocadlo, D. J. ; Withers, S. G. How To Make A Difference: Mechanisms Of Protein And Nucleic Acid Modifying Enzymes Editorial Overview. CURRENT OPINION IN CHEMICAL BIOLOGY 2012, 16, 461-464.
Withers, S. G. ; Davies, G. J. Glycobiology The Case Of The Missing Base. NATURE CHEMICAL BIOLOGY 2012, 8, 952-953.
Williams, L. K. ; Li, C. ; Withers, S. G. ; Brayer, G. D. Order And Disorder: Differential Structural Impacts Of Myricetin And Ethyl Caffeate On Human Amylase, An Antidiabetic Target. JOURNAL OF MEDICINAL CHEMISTRY 2012, 55, 10177-10186.
Reitinger, S. ; Petriv, O. I. ; Mehr, K. ; Hansen, C. L. ; Withers, S. G. Purification And Quantitation Of Bacteriophage M13 Using Desalting Spin Columns And Digital Pcr. JOURNAL OF VIROLOGICAL METHODS 2012, 185, 171-174.
Shim, J. - H. ; Chen, H. - M. ; Rich, J. R. ; Goddard-Borger, E. D. ; Withers, S. G. Directed Evolution Of A Beta-Glycosidase From Agrobacterium Sp. To Enhance Its Glycosynthase Activity Toward C3-Modified Donor Sugars. PROTEIN ENGINEERING DESIGN & SELECTION 2012, 25, 465-472.
Pengthaisong, S. ; Chen, C. - F. ; Withers, S. G. ; Kuaprasert, B. ; Cairns, J. R. Ketudat Rice Bglu1 Glycosynthase And Wild Type Transglycosylation Activities Distinguished By Cyclophellitol Inhibition. CARBOHYDRATE RESEARCH 2012, 352, 51-59.
Michikawa, M. ; Ichinose, H. ; Momma, M. ; Biely, P. ; Jongkees, S. ; Yoshida, M. ; Kotake, T. ; Tsumuraya, Y. ; Withers, S. G. ; Fujimoto, Z. ; Kaneko, S. Structural And Biochemical Characterization Of Glycoside Hydrolase Family 79 Beta-Glucuronidase From Acidobacterium Capsulatum. JOURNAL OF BIOLOGICAL CHEMISTRY 2012, 287, 14069-14077.
Goddard-Borger, E. D. ; Tropak, M. B. ; Yonekawa, S. ; Tysoe, C. ; Mahuran, D. J. ; Withers, S. G. Rapid Assembly Of A Library Of Lipophilic Iminosugars Via The Thiol-Ene Reaction Yields Promising Pharmacological Chaperones For The Treatment Of Gaucher Disease. JOURNAL OF MEDICINAL CHEMISTRY 2012, 55, 2737-2745.
Pengthaisong, S. ; Withers, S. G. ; Kuaprasert, B. ; Svasti, J. ; Cairns, J. R. Ketudat The Role Of The Oligosaccharide Binding Cleft Of Rice Bglu1 In Hydrolysis Of Cellooligosaccharides And In Their Synthesis By Rice Bglu1 Glycosynthase. PROTEIN SCIENCE 2012, 21, 362-372.
Goddard-Borger, E. D. ; Sakaguchi, K. ; Reitinger, S. ; Watanabe, N. ; Ito, M. ; Withers, S. G. Mechanistic Insights Into The 1,3-Xylanases: Useful Enzymes For Manipulation Of Algal Biomass. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2012, 134, 3895-3902.
Rich, J. R. ; Withers, S. G. A Chemoenzymatic Total Synthesis Of The Neurogenic Starfish Ganglioside Llg-3 Using An Engineered And Evolved Synthase. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2012, 51, 8640-8643.

2011

Kwan, D. H. ; Withers, S. G. Toward Efficient Enzymatic Glycan Synthesis: Directed Evolution And Enzyme Engineering. JOURNAL OF CARBOHYDRATE CHEMISTRY 2011, 30, 181-205.
Lin, L. Y. - C. ; Rakic, B. ; Chiu, C. P. C. ; Lameignere, E. ; Wakarchuk, W. W. ; Withers, S. G. ; Strynadka, N. C. J. Structure And Mechanism Of The Lipooligosaccharide Sialyltransferase From Neisseria Meningitidis. JOURNAL OF BIOLOGICAL CHEMISTRY 2011, 286, 37237-37248.
Zhang, R. ; Pan, Y. T. ; He, S. ; Lam, M. ; Brayer, G. D. ; Elbein, A. D. ; Withers, S. G. Mechanistic Analysis Of Trehalose Synthase From Mycobacterium Smegmatis. JOURNAL OF BIOLOGICAL CHEMISTRY 2011, 286, 35601-35609.
Lee, H. Jun; Lairson, L. L. ; Rich, J. R. ; Lameignere, E. ; Wakarchuk, W. W. ; Withers, S. G. ; Strynadka, N. C. J. Structural And Kinetic Analysis Of Substrate Binding To The Sialyltransferase Cst-Ii From Campylobacter Jejuni. JOURNAL OF BIOLOGICAL CHEMISTRY 2011, 286, 35922-35932.
Lee, S. Seo; Greig, I. R. ; Vocadlo, D. J. ; McCarter, J. D. ; Patrick, B. O. ; Withers, S. G. Structural, Mechanistic, And Computational Analysis Of The Effects Of Anomeric Fluorines On Anomeric Fluoride Departure In 5-Fluoroxylosyl Fluorides. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2011, 133, 15826-15829.
Torpenholt, S. ; Le Nours, J. ; Christensen, U. ; Jahn, M. ; Withers, S. G. ; Ostergaard, P. Rahbek; Borchert, T. V. ; Poulsen, J. - C. ; Leggio, L. Lo Activity Of Three Beta-1,4-Galactanases On Small Chromogenic Substrates. CARBOHYDRATE RESEARCH 2011, 346, 2028-2033.
Hill, T. ; Tropak, M. B. ; Mahuran, D. ; Withers, S. G. Synthesis, Kinetic Evaluation And Cell-Based Analysis Of C-Alkylated Isofagomines As Chaperones Of Beta-Glucocerebrosidase. CHEMBIOCHEM 2011, 12, 2151-2154.
Froehlich, R. F. G. ; Furneaux, R. H. ; Mahuran, D. J. ; Saf, R. ; Stuetz, A. E. ; Tropak, M. B. ; Wicki, J. ; Withers, S. G. ; Wrodnigg, T. M. 1-Deoxy-D-Galactonojirimycins With Dansyl Capped N-Substituents As Beta-Galactosidase Inhibitors And Potential Probes For G(M1) Gangliosidosis Affected Cell Lines. CARBOHYDRATE RESEARCH 2011, 346, 1592-1598.
Goddard-Borger, E. D. ; Fiege, B. ; Kwan, E. M. ; Withers, S. G. Glycosynthase-Mediated Assembly Of Xylanase Substrates And Inhibitors. CHEMBIOCHEM 2011, 12, 1703-1711.
Kwan, D. H. ; Chen, H. - M. ; Ratananikom, K. ; Hancock, S. M. ; Watanabe, Y. ; Kongsaeree, P. T. ; A. Samuels, L. ; Withers, S. G. Self-Immobilizing Fluorogenic Imaging Agents Of Enzyme Activity. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2011, 50, 300-303.
Rempel, B. P. ; Tropak, M. B. ; Mahuran, D. J. ; Withers, S. G. Tailoring The Specificity And Reactivity Of A Mechanism-Based Inactivator Of Glucocerebrosidase For Potential Therapeutic Applications. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2011, 50, 10381-10383.
Rich, J. R. ; Cunningham, A. - M. ; Gilbert, M. ; Withers, S. G. Glycosphingolipid Synthesis Employing A Combination Of Recombinant Glycosyltransferases And An Endoglycoceramidase Glycosynthase. CHEMICAL COMMUNICATIONS 2011, 47, 10806-10808.
Jongkees, S. A. K. ; Withers, S. G. Glycoside Cleavage By A New Mechanism In Unsaturated Glucuronyl Hydrolases. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2011, 133, 19334-19337.
Win-Mason, A. L. ; Jongkees, S. A. K. ; Withers, S. G. ; Tyler, P. C. ; Timmer, M. S. M. ; Stocker, B. L. Stereoselective Total Synthesis Of Aminoiminohexitols Via Carbamate Annulation. JOURNAL OF ORGANIC CHEMISTRY 2011, 76, 9611-9621.
Froehlich, R. F. G. ; Fantur, K. ; Furneaux, R. H. ; Paschke, E. ; Stuetz, A. E. ; Wicki, J. ; Withers, S. G. ; Wrodnigg, T. M. A Fluorescent Probe For Gm1 Gangliosidosis Related Beta-Galactosidase: N-(Dansylamino) Hexylaminocarbonylpentyl-1,5-Dideoxy-1,5-Imino-D-Galactitol. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 2011, 21, 6872-6875.

2010

Chen, H. - M. ; Withers, S. G. Syntheses Of P-Nitrophenyl 3-And 4-Thio-Beta-D-Glycopyranosides. CARBOHYDRATE RESEARCH 2010, 345, 2596-2604.
Goddard-Borger, E. D. ; Wennekes, T. ; Withers, S. G. Molecular Probes Getting Lucky In The Lysosome. NATURE CHEMICAL BIOLOGY 2010, 6, 881-883.
Kim, E. Ju; Love, D. C. ; Darout, E. ; Abdo, M. ; Rempel, B. ; Withers, S. G. ; Rablen, P. R. ; Hanover, J. A. ; Knapp, S. Oga Inhibition By Glcnac-Selenazoline. BIOORGANIC & MEDICINAL CHEMISTRY 2010, 18, 7058-7064.
Schitter, G. ; Steiner, A. J. ; Pototschnig, G. ; Scheucher, E. ; Thonhofer, M. ; Tarling, C. A. ; Withers, S. G. ; Fantur, K. ; Paschke, E. ; Mahuran, D. J. ; Rigat, B. A. ; Tropak, M. B. ; Illaszewicz, C. ; Saf, R. ; Stuetz, A. E. ; Wrodnigg, T. M. Fluorous Iminoalditols: A New Family Of Glycosidase Inhibitors And Pharmacological Chaperones. CHEMBIOCHEM 2010, 11, 2026-2033.
Yang, G. ; Rich, J. R. ; Gilbert, M. ; Wakarchuk, W. W. ; Feng, Y. ; Withers, S. G. Fluorescence Activated Cell Sorting As A General Ultra-High-Throughput Screening Method For Directed Evolution Of Glycosyltransferases. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2010, 132, 10570-10577.
Pototschnig, G. ; De Csaky, C. Morales; Burke, J. R. Montene; Schitter, G. ; Stuetz, A. E. ; Tarling, C. A. ; Withers, S. G. ; Wrodnigg, T. M. Synthesis And Biological Evaluation Of Novel Biotin-Iminoalditol Conjugates. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 2010, 20, 4077-4079.
Morley, T. J. ; Withers, S. G. Chemoenzymatic Synthesis And Enzymatic Analysis Of 8-Modified Cytidine Monophosphate-Sialic Acid And Sialyl Lactose Derivatives. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2010, 132, 9430-9437.
Kittl, R. ; Withers, S. G. New Approaches To Enzymatic Glycoside Synthesis Through Directed Evolution. CARBOHYDRATE RESEARCH 2010, 345, 1272-1279.
Froehlich, R. F. G. ; Furneaux, R. H. ; Mahuran, D. J. ; Rigat, B. A. ; Stuetz, A. E. ; Tropak, M. B. ; Wicki, J. ; Withers, S. G. ; Wrodnigg, T. M. 1-Deoxynojirimycins With Dansyl Capped N-Substituents As Probes For Morbus Gaucher Affected Cell Lines. CARBOHYDRATE RESEARCH 2010, 345, 1371-1376.
Phenix, C. P. ; Rempel, B. P. ; Colobong, K. ; Doudet, D. J. ; Adam, M. J. ; Clarke, L. A. ; Withers, S. G. Imaging Of Enzyme Replacement Therapy Using Pet. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2010, 107, 10842-10847.
Reitinger, S. ; Yu, Y. ; Wicki, J. ; Ludwiczek, M. ; D'Angelo, I. ; Baturin, S. ; Okon, M. ; Strynadka, N. C. J. ; Lutz, S. ; Withers, S. G. ; McIntosh, L. P. Circular Permutation Of Bacillus Circulans Xylanase: A Kinetic And Structural Study. BIOCHEMISTRY 2010, 49, 2464-2474.
Schitter, G. ; Scheucher, E. ; Steiner, A. J. ; Stuetz, A. E. ; Thonhofer, M. ; Tarling, C. A. ; Withers, S. G. ; Wicki, J. ; Fantur, K. ; Paschke, E. ; Mahuran, D. J. ; Rigat, B. A. ; Tropak, M. ; Wrodnigg, T. M. Synthesis Of Lipophilic 1-Deoxygalactonojirimycin Derivatives As D-Galactosidase Inhibitors. BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY 2010, 6, 21.
Macdonald, J. M. ; Tarling, C. A. ; Taylor, E. J. ; Dennis, R. J. ; Myers, D. S. ; Knapp, S. ; Davies, G. J. ; Withers, S. G. Chitinase Inhibition By Chitobiose And Chitotriose Thiazolines. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2010, 49, 2599-2602.
Kim, Y. - W. ; Zhang, R. ; Chen, H. ; Withers, S. G. O-Glycoligases, A New Category Of Glycoside Bond-Forming Mutant Glycosidases, Catalyse Facile Syntheses Of Isoprimeverosides. CHEMICAL COMMUNICATIONS 2010, 46, 8725-8727.

2009

Chan, P. H. W. ; Lairson, L. L. ; Lee, H. Jun; Wakarchuk, W. W. ; Strynadka, N. C. J. ; Withers, S. G. ; McIntosh, L. P. Nmr Spectroscopic Characterization Of The Sialyltransferase Cstii From Campylobacter Jejuni: Histidine 188 Is The General Base. BIOCHEMISTRY 2009, 48, 11220-11230.
Rempel, B. P. ; Withers, S. G. Non-Stick Sugars: Synthesis Of Difluorosugar Fluorides As Potential Glycosidase Inactivators. AUSTRALIAN JOURNAL OF CHEMISTRY 2009, 62, 590-599.
Yang, G. ; Withers, S. G. Ultrahigh-Throughput Facs-Based Screening For Directed Enzyme Evolution. CHEMBIOCHEM 2009, 10, 2704-2715.
Offen, W. A. ; Zechel, D. L. ; Withers, S. G. ; Gilbert, H. J. ; Davies, G. J. Structure Of The Michaelis Complex Of Beta-Mannosidase, Man2A, Provides Insight Into The Conformational Itinerary Of Mannoside Hydrolysis. CHEMICAL COMMUNICATIONS 2009, 2484-2486.
Zhang, R. ; Li, C. ; Williams, L. K. ; Rempel, B. P. ; Brayer, G. D. ; Withers, S. G. Directed ``in Situ'' Inhibitor Elongation As A Strategy To Structurally Characterize The Covalent Glycosyl-Enzyme Intermediate Of Human Pancreatic Alpha-Amylase. BIOCHEMISTRY 2009, 48, 10752-10764.
Willis, L. M. ; Zhang, R. ; Reid, A. ; Withers, S. G. ; Wakarchuk, W. W. Mechanistic Investigation Of The Endo-Alpha-N-Acetylgalactosaminidase From Streptococcus Pneumoniae R6. BIOCHEMISTRY 2009, 48, 10334-10341.
Rao, F. V. ; Rich, J. R. ; Rakic, B. ; Buddai, S. ; Schwartz, M. F. ; Johnson, K. ; Bowe, C. ; Wakarchuk, W. W. ; DeFrees, S. ; Withers, S. G. ; Strynadka, N. C. J. Structural Insight Into Mammalian Sialyltransferases. NATURE STRUCTURAL & MOLECULAR BIOLOGY 2009, 16, 1186-1188.
Kim, J. Hyo; Ryu, H. Won; Shim, J. Hoon; Park, K. Hun; Withers, S. G. Development Of New And Selective Trypanosoma Cruzi Trans-Sialidase Inhibitors From Sulfonamide Chalcones And Their Derivatives. CHEMBIOCHEM 2009, 10, 2475-2479.
Shaikh, F. Aidha; Randriantsoa, M. ; Withers, S. G. Mechanistic Analysis Of The Blood Group Antigen-Cleaving Endo-Beta-Galactosidase From Clostridium Perfringens. BIOCHEMISTRY 2009, 48, 8396-8404.
Lee, H. Jun; Rakic, B. ; Gilbert, M. ; Wakarchuk, W. W. ; Withers, S. G. ; Strynadka, N. C. J. Structural And Kinetic Characterizations Of The Polysialic Acid O-Acetyltransferase Oatwy From Neisseria Meningitidis. JOURNAL OF BIOLOGICAL CHEMISTRY 2009, 284, 24501-24511.
Hancock, S. M. ; Rich, J. R. ; Caines, M. E. C. ; Strynadka, N. C. J. ; Withers, S. G. Designer Enzymes For Glycosphingolipid Synthesis By Directed Evolution. NATURE CHEMICAL BIOLOGY 2009, 5, 508-514.
Morley, T. J. ; Willis, L. M. ; Whitfield, C. ; Wakarchuk, W. W. ; Withers, S. G. A New Sialidase Mechanism Bacteriophage K1F Endo-Sialidase Is An Inverting Glycosidase. JOURNAL OF BIOLOGICAL CHEMISTRY 2009, 284, 17404-17410.
Steiner, A. J. ; Schitter, G. ; Stuetz, A. E. ; Wrodnigg, T. M. ; Tarling, C. A. ; Withers, S. G. ; Mahuran, D. J. ; Tropak, M. B. 2-Acetamino-1,2-Dideoxynojirimycin-Lysine Hybrids As Hexosaminidase Inhibitors. TETRAHEDRON-ASYMMETRY 2009, 20, 832-835.
Rich, J. R. ; Withers, S. G. Emerging Methods For The Production Of Homogeneous Human Glycoproteins. NATURE CHEMICAL BIOLOGY 2009, 5, 206-215.
Le, Q. - T. ; Lee, C. - K. ; Kim, Y. - W. ; Lee, S. - J. ; Zhang, R. ; Withers, S. G. ; Kim, Y. - R. ; Auh, J. - H. ; Park, K. - H. Amylolytically-Resistant Tapioca Starch Modified By Combined Treatment Of Branching Enzyme And Maltogenic Amylase. CARBOHYDRATE POLYMERS 2009, 75, 9-14.
Rakic, B. ; Withers, S. G. Recent Developments In Glycoside Synthesis With Glycosynthases And Thioglycoligases. AUSTRALIAN JOURNAL OF CHEMISTRY 2009, 62, 510-520.
Davies, G. J. ; Withers, S. G. ; Vocadlo, D. J. The Chitopentaose Complex Of A Mutant Hen Egg-White Lysozyme Displays No Distortion Of The-1 Sugar Away From A C-4(1) Chair Conformation. AUSTRALIAN JOURNAL OF CHEMISTRY 2009, 62, 528-532.
Steiner, A. J. ; Stuetz, A. E. ; Tarling, C. A. ; Withers, S. G. ; Wrodnigg, T. M. Synthesis And Biological Evaluation Of 1,5-Dideoxy-1,5-Iminoxylitol-Amino Acid Hybrids As Xylosidase Inhibitors. AUSTRALIAN JOURNAL OF CHEMISTRY 2009, 62, 553-557.

2008

Greig, I. R. ; Zahariev, F. ; Withers, S. G. Elucidating The Nature Of The Streptomyces Plicatus Beta-Hexosaminidase-Bound Intermediate Using Ab Initio Molecular Dynamics Simulations. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2008, 130, 17620-17628.
Steiner, A. J. ; Schitter, G. ; Stuetz, A. E. ; Wrodnigg, T. M. ; Tarling, C. A. ; Withers, S. G. ; Fantur, K. ; Mahuran, D. ; Paschke, E. ; Tropak, M. 1-Deoxygalactonojirimycin-Lysine Hybrids As Potent D-Galactosidase Inhibitors. BIOORGANIC & MEDICINAL CHEMISTRY 2008, 16, 10216-10220.
Caines, M. E. C. ; Zhu, H. ; Vuckovic, M. ; Willis, L. M. ; Withers, S. G. ; Wakarchuk, W. W. ; Strynadka, N. C. J. The Structural Basis For T-Antigen Hydrolysis By Streptococcus Pneumoniae A Target For Structure-Based Vaccine Design. JOURNAL OF BIOLOGICAL CHEMISTRY 2008, 283, 31279-31283.
Hancock, S. M. ; Tarling, C. A. ; Withers, S. G. High-Throughput Screening Of Cell Lysates For Ganglioside Synthesis. ANALYTICAL BIOCHEMISTRY 2008, 382, 48-54.
Kuntz, D. A. ; Tarling, C. A. ; Withers, S. G. ; Rose, D. R. Structural Analysis Of Golgi Alpha-Mannosidase Ii Inhibitors Identified From A Focused Glycosidase Inhibitor Screen. BIOCHEMISTRY 2008, 47, 10058-10068.
Rempel, B. P. ; Withers, S. G. Covalent Inhibitors Of Glycosidases And Their Applications In Biochemistry And Biology. GLYCOBIOLOGY 2008, 18, 570-586.
Hekmat, O. ; He, S. ; R. Warren, A. J. ; Withers, S. G. A Mechanism-Based Icat Strategy For Comparing Relative Expression And Activity Levels Of Glycosidases In Biological Systems. JOURNAL OF PROTEOME RESEARCH 2008, 7, 3282-3292.
Lee, Y. - S. ; Lee, M. - H. ; Lee, H. - S. ; Lee, S. - J. ; Kim, Y. - W. ; Zhang, R. ; Withers, S. G. ; Kim, K. Soo; Lee, S. - J. ; Park, K. - H. Enzymatic Synthesis Of A Selective Inhibitor For Alpha-Glucosidases: Alpha-Acarviosinyl-(1 -> 9)-3-Alpha-D-Glucopyranosylpropen. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2008, 56, 5324-5330.
Namdjou, D. - J. ; Chen, H. - M. ; Vinogradov, ii, E. ; Brochu, D. ; Withers, S. G. ; Wakarchuk, W. W. A Beta-1,4-Galactosyltransferase From Helicobacter Pylori Is An Efficient And Versatile Biocatalyst Displaying A Novel Activity For Thioglycoside Synthesis. CHEMBIOCHEM 2008, 9, 1632-1640.
Amorelli, B. ; Yang, C. ; Rempel, B. ; Withers, S. G. ; Knapp, S. N-Acetylhexosaminidase Inhibitory Properties Of C-1 Homologated Glcnac- And Galnac-Thiazolines. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 2008, 18, 2944-2947.
Zhang, R. ; McCarter, J. D. ; Braun, C. ; Yeung, W. ; Brayer, G. D. ; Withers, S. G. Synthesis And Testing Of 2-Deoxy-2,2-Dihaloglycosides As Mechanism-Based Inhibitors Of Alpha-Glycosidases. JOURNAL OF ORGANIC CHEMISTRY 2008, 73, 3070-3077.
Wilkinson, S. M. ; Liew, C. W. ; Mackay, J. P. ; Salleh, H. M. ; Withers, S. G. ; McLeod, M. D. Escherichia Coli Glucuronylsynthase: An Engineered Enzyme For The Synthesis Of Beta-Glucuronides. ORGANIC LETTERS 2008, 10, 1585-1588.

2007

Meloncelli, P. J. ; Gloster, T. M. ; Money, V. A. ; Tarling, C. A. ; Davies, G. J. ; Withers, S. G. ; Stick, R. V. D-Glucosylated Derivatives Of Isofagomine And Noeuromycin And Their Potential As Inhibitors Of Beta-Glycoside Hydrolases. AUSTRALIAN JOURNAL OF CHEMISTRY 2007, 60, 549-565.
Lairson, L. L. ; Wakarchuk, W. W. ; Withers, S. G. Alternative Donor Substrates For Inverting And Retaining Glycosyltransferases. CHEMICAL COMMUNICATIONS 2007, 365-367.
Tesic, M. ; Wicki, J. ; Poon, D. K. Y. ; Withers, S. G. ; Douglas, D. J. Gas Phase Noncovalent Protein Complexes That Retain Solution Binding Properties: Binding Of Xylobiose Inhibitors To The Beta-1,4 Exoglucanase From Cellulomonas Fimi. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 2007, 18, 64-73.

2006

Greimel, P. ; Hausler, H. ; Lundt, I. ; RUPITZ, K. ; Stutz, A. E. ; Tarling, C. A. ; Withers, S. G. ; Wrodnigg, T. M. Fluorescent Glycosidase Inhibiting 1,5-Dideoxy-1,5-Iminoalditols. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 2006, 16, 2067-2070.
Rye, C. S. ; Matte, A. ; Cygler, M. ; Withers, S. G. An Atypical Approach Identifies Tyr234 As The Key Base Catalyst In Chondroitin Ac Lyase. CHEMBIOCHEM 2006, 7, 631-637.
Yip, V. L. ; Withers, S. G. Breakdown Of Oligosaccharides By The Process Of Elimination. CURRENT OPINION IN CHEMICAL BIOLOGY 2006, 10, 147-155.
Poon, D. K. Y. ; Schubert, M. ; Au, J. ; Okon, M. ; Withers, S. G. ; McIntosh, L. P. Unambiguous Determination Of The Ionization State Of A Glycoside Hydrolase Active Site Lysine By H-1-N-15 Heteronuclear Correlation Spectroscopy. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2006, 128, 15388-15389.
Lundt, I. ; Steiner, A. J. ; Stutz, A. E. ; Tarling, C. A. ; Ully, S. ; Withers, S. G. ; Wrodnigg, T. M. Fluorescently Tagged Iminoalditol Glycosidase Inhibitors As Novel Biological Probes And Diagnostics. BIOORGANIC & MEDICINAL CHEMISTRY 2006, 14, 1737-1742.
Lairson, L. L. ; Watts, A. G. ; Wakarchuk, W. W. ; Withers, S. G. Using Substrate Engineering To Harness Enzymatic Promiscuity And Expand Biological Catalysis. NATURE CHEMICAL BIOLOGY 2006, 2, 724-728.
Kim, Y. W. ; Lovering, A. L. ; Chen, H. M. ; Kantner, T. ; McIntosh, L. P. ; Strynadka, N. C. J. ; Withers, S. G. Expanding The Thioglycoligase Strategy To The Synthesis Of Alpha-Linked Thioglycosides Allows Structural Investigation Of The Parent Enzyme/substrate Complex. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2006, 128, 2202-2203.
Hancock, S. M. ; Vaughan, M. D. ; Withers, S. G. Engineering Of Glycosidases And Glycosyltransferases. CURRENT OPINION IN CHEMICAL BIOLOGY 2006, 10, 509-519.
Watts, A. G. ; Oppezzo, P. ; Withers, S. G. ; Alzari, P. M. ; Buschiazzo, A. Structural And Kinetic Analysis Of Two Covalent Sialosyl-Enzyme Intermediates On Trypanosoma Rangeli Sialidase. JOURNAL OF BIOLOGICAL CHEMISTRY 2006, 281, 4149-4155.
Blair, D. E. ; Hekmat, O. ; Schuttelkopf, A. W. ; Shrestha, B. ; Tokuyasu, K. ; Withers, S. G. ; van Aalten, D. M. F. Structure And Mechanism Of Chitin Deacetylase From The Fungal Pathogen Colletotrichum Lindemuthianum. BIOCHEMISTRY 2006, 45, 9416-9426.
Tauss, A. ; Steiner, A. J. ; Stutz, A. E. ; Tarling, C. A. ; Withers, S. G. ; Wrodnigg, T. M. L-Idoseptanosides: Substratesd D-Glucosidases?. TETRAHEDRON-ASYMMETRY 2006, 17, 234-239.
Aharoni, A. ; Thieme, K. ; Chiu, C. P. C. ; Buchini, S. ; Lairson, L. L. ; Chen, H. ; Strynadka, N. C. J. ; Wakarchuk, W. W. ; Withers, S. G. High-Throughput Screening Methodology For The Directed Evolution Of Glycosyltransferases. NATURE METHODS 2006, 3, 609-614.
Salleh, H. M. ; Mullegger, J. ; Reid, S. P. ; Chan, W. Y. ; Hwang, J. Y. ; WARREN, R. A. J. ; Withers, S. G. Cloning And Characterization Of Thermotoga Maritima Beta-Glucuronidase. CARBOHYDRATE RESEARCH 2006, 341, 49-59.
Wrodnigg, T. M. ; Stuetz, A. E. ; Tarling, C. A. ; Withers, S. G. Fine Tuning Of Beta-Glucosidase Inhibitory Activity In The 2,5-Dideoxy-2,5-Imino-D-Mannitol (Dmdp) System. CARBOHYDRATE RESEARCH 2006, 341, 1717-1722.
Mullegger, J. ; Chen, H. M. ; WARREN, R. A. J. ; Withers, S. G. Glycosylation Of A Neoglycoprotein By Using Glycosynthase And Thioglycoligase Approaches: The Generation Of A Thioglycoprotein. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2006, 45, 2585-2588.
Mullegger, J. ; Chen, H. - M. ; Chan, W. Yiu; Reid, S. P. ; Jahn, M. ; R. Warren, A. J. ; Salleh, H. Mohammed; Withers, S. G. Thermostable Glycosynthases And Thioglycoligases Derived From Thermotoga Maritima Beta-Glucuronidase. CHEMBIOCHEM 2006, 7, 1028-1030.
Yip, V. L. Y. ; Withers, S. G. Family 4 Glycosidases Carry Out Efficient Hydrolysis Of Thioglycosides By An Alpha,beta-Elimination Mechanism. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2006, 45, 6179-6182.
Mayer, C. ; Vocadlo, D. J. ; Mah, M. ; RUPITZ, K. ; Stoll, D. ; WARREN, R. A. J. ; Withers, S. G. Characterization Of A Beta-N-Acetylhexosaminidase And A Beta-N-Acetylglucosaminidase/beta-Glucosidase From Cellulomonas Fimi. FEBS JOURNAL 2006, 273, 2929-2941.
Yip, V. L. Y. ; Withers, S. G. Family 4 Glycoside Hydrolases Are Special: The First Beta-Elimination Mechanism Amongst Glycoside Hydrolases. BIOCATALYSIS AND BIOTRANSFORMATION 2006, 24, 167-176.
Gordon, R. D. ; Sivarajah, P. ; Satkunarajah, M. ; Ma, D. ; Tarling, C. A. ; Vizitiu, D. ; Withers, S. G. ; Rini, J. M. X-Ray Crystal Structures Of Rabbit N-Acetylglucosaminyltransferase I (Gnt I) In Complex With Donor Substrate Analogues. JOURNAL OF MOLECULAR BIOLOGY 2006, 360, 67-79.
Williams, S. J. ; Hekmat, O. ; Withers, S. G. Synthesis And Testing Of Mechanism-Based Protein-Profiling Probes For Retaining Endo-Glycosidases. CHEMBIOCHEM 2006, 7, 116-124.
Lemieux, M. J. ; Mark, B. L. ; Cherney, M. M. ; Withers, S. G. ; Mahuran, D. J. ; James, M. N. G. Crystallographic Structure Of Human Beta-Hexosaminidase A: Interpretation Of Tay-Sachs Mutations And Loss Of G(M2) Ganglioside Hydrolysis. JOURNAL OF MOLECULAR BIOLOGY 2006, 359, 913-929.
Pabba, J. ; Rempel, B. P. ; Withers, S. G. ; VASELLA, A. Synthesis Of Glycaro-1,5-Lactams And Tetrahydrotetrazolopyridine-5-Carboxylates: Inhibitors Of Beta-D-Glucuronidase And Alpha-L-Iduronidase. HELVETICA CHIMICA ACTA 2006, 89, 635-666.
Mildvan, A. S. ; Xia, Z. ; Azurmendi, H. F. ; Legler, P. M. ; Balfour, M. R. ; Lairson, L. L. ; Withers, S. G. ; Gabelli, S. B. ; Bianchet, M. A. ; Amzel, L. M. Hydrogen Bonding In The Mechanism Of Gdp-Mannose Mannosyl Hydrolase. JOURNAL OF MOLECULAR STRUCTURE 2006, 790, 160-167.
Kim, Y. W. ; Fox, D. T. ; Hekmat, O. ; Kantner, T. ; McIntosh, L. P. ; WARREN, R. A. J. ; Withers, S. G. Glycosynthase-Based Synthesis Of Xylo-Oligosaccharides Using An Engineered Retaining Xylanase From Cellulomonas Fimi. ORGANIC & BIOMOLECULAR CHEMISTRY 2006, 4, 2025-2032.
Vaughan, M. D. ; Johnson, K. ; DeFrees, S. ; Tang, X. ; R. Warren, A. J. ; Withers, S. G. Glycosynthase-Mediated Synthesis Of Glycosphingolipids. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2006, 128, 6300-6301.

2005

Kim, Y. W. ; Chen, H. M. ; Withers, S. G. Enzymatic Transglycosylation Of Xylose Using A Glycosynthase. CARBOHYDRATE RESEARCH 2005, 340, 2735-2741.
Duncan, G. ; Tarling, C. A. ; Ringle, W. H. ; Nomellini, J. F. ; Yamage, M. ; Dorocicz, I. R. ; Withers, S. G. ; Smit, J. Evaluation Of A New System For Developing Particulate Enzymes Based On The Surface (S)-Layer Protein (Rsaa) Of Caulobacter Crescentus - Fusion With The Beta-1,4-Glycanase (Cex) From The Cellulolytic Bacterium Cellulomonas Fimi Yields A Robust, Catalytical. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2005, 127, 95-110.
Hekmat, O. ; Kim, Y. W. ; Williams, S. J. ; He, S. M. ; Withers, S. G. Active-Site Peptide ``fingerprinting'' Of Glycosidases In Complex Mixtures By Mass Spectrometry - Discovery Of A Novel Retaining Beta-1,4-Glycanase In Cellulomonas Fimi. JOURNAL OF BIOLOGICAL CHEMISTRY 2005, 280, 35126-35135.
Knapp, S. ; Yang, C. H. ; Pabbaraja, S. ; Rempel, B. ; Reid, S. ; Withers, S. G. Synthesis And Kinetic Analysis Of The N-Acetylhexosaminidase Inhibitor Xylnac-Isofagomine. JOURNAL OF ORGANIC CHEMISTRY 2005, 70, 7715-7720.
Xia, Z. Y. ; Azurmendi, H. F. ; Lairson, L. L. ; Withers, S. G. ; Gabelli, S. B. ; Bianchet, M. A. ; Amzel, L. M. ; Mildvan, A. S. Mutational, Structural, And Kinetic Evidence For A Dissociative Mechanism In The Gdp-Mannose Mannosyl Hydrolase Reaction. BIOCHEMISTRY 2005, 44, 8989-8997.
Rempel, B. P. ; Clarke, L. A. ; Withers, S. G. A Homology Model For Human Alpha-L-Iduronidase: Insights Into Human Disease. MOLECULAR GENETICS AND METABOLISM 2005, 85, 28-37.
Maurus, R. ; Begum, A. ; Kuo, H. H. ; Racaza, A. ; Numao, S. ; Andersen, C. ; Tams, J. P. ; Vind, J. ; Overall, C. M. ; Withers, S. G. ; BRAYER, G. D. Structural And Mechanistic Studies Of Chloride Induced Activation Of Human Pancreatic Alpha-Amylase. PROTEIN SCIENCE 2005, 14, 743-755.
Vocadlo, D. J. ; Withers, S. G. The Chemical Synthesis Of 2-Deoxy-2-Fluorodisaccharide Probes Of The Hen Egg White Lysozyme Mechanism. CARBOHYDRATE RESEARCH 2005, 340, 379-388.
Varrot, A. ; Yip, V. L. Y. ; Li, Y. S. ; Rajan, S. S. ; Yang, X. J. ; Anderson, W. F. ; Thompson, J. ; Withers, S. G. ; Davies, G. J. Nad(+) And Metal-Ion Dependent Hydrolysis By Family 4 Glycosidases: Structural Insight Into Specificity For Phospho-Beta-D-Glucosides. JOURNAL OF MOLECULAR BIOLOGY 2005, 346, 423-435.
Lovering, A. L. ; Lee, S. S. ; Kim, Y. W. ; Withers, S. G. ; Strynadka, N. C. J. Mechanistic And Structural Analysis Of A Family 31 Alpha-Glycosidase And Its Glycosyl-Enzyme Intermediate. JOURNAL OF BIOLOGICAL CHEMISTRY 2005, 280, 2105-2115.
Tauss, A. ; Greimel, P. ; RUPITZ, K. ; Steiner, A. J. ; Stutz, A. E. ; Withers, S. G. ; Wrodnigg, T. M. Non-Natural Aldofuranosides As Substrates Of A Beta-Glucosidase. TETRAHEDRON-ASYMMETRY 2005, 16, 159-165.
Lee, S. S. ; Yu, S. K. ; Withers, S. G. Mechanism Of Action Of Exo-Acting Alpha-1,4-Glucan Lyase: A Glycoside Hydrolase Family 31 Enzyme. BIOLOGIA 2005, 60, 137-148.
Mullegger, J. ; Jahn, M. ; Chen, H. M. ; WARREN, R. A. J. ; Withers, S. G. Engineering Of A Thioglycoligase: Randomized Mutagenesis Of The Acid-Base Residue Leads To The Identification Of Improved Catalysts. PROTEIN ENGINEERING DESIGN & SELECTION 2005, 18, 33-40.

2004

Numao, S. ; Damager, I. ; Li, C. M. ; Wrodnigg, T. M. ; Begum, A. ; Overall, C. M. ; BRAYER, G. D. ; Withers, S. G. In Situ Extension As An Approach For Identifying Novel Alpha-Amylase Inhibitors. JOURNAL OF BIOLOGICAL CHEMISTRY 2004, 279, 48282-48291.
Watts, A. G. ; Withers, S. G. The Synthesis Of Some Mechanistic Probes For Sialic Acid Processing Enzymes And The Labeling Of A Sialidase From Trypanosoma Rangeli. CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE 2004, 82, 1581-1588.
Lairson, L. L. ; Withers, S. G. Mechanistic Analogies Amongst Carbohydrate Modifying Enzymes. CHEMICAL COMMUNICATIONS 2004, 2243-2248.
Chiu, C. P. C. ; Watts, A. G. ; Lairson, L. L. ; Gilbert, M. ; Lim, D. ; WAKARCHUK, W. W. ; Withers, S. G. ; Strynadka, N. C. J. Structural Analysis Of The Sialyltransferase Cstii From Campylobacter Jejuni In Complex With A Substrate Analog. NATURE STRUCTURAL & MOLECULAR BIOLOGY 2004, 11, 163-170.
Kim, Y. W. ; Lee, S. S. ; WARREN, R. A. J. ; Withers, S. G. Directed Evolution Of A Glycosynthase From Agrobacterium Sp Increases Its Catalytic Activity Dramatically And Expands Its Substrate Repertoire. JOURNAL OF BIOLOGICAL CHEMISTRY 2004, 279, 42787-42793.
Gibson, R. P. ; Tarling, C. A. ; Roberts, S. ; Withers, S. G. ; Davies, G. J. The Donor Subsite Of Trehalose-6-Phosphate Synthase - Binary Complexes With Udp-Glucose And Udp-2-Deoxy-2-Fluoro-Glucose At 2 Angstrom Resolution. JOURNAL OF BIOLOGICAL CHEMISTRY 2004, 279, 1950-1955.
Rajan, S. S. ; Yang, X. J. ; Collart, F. ; Yip, V. L. Y. ; Withers, S. G. ; Varrot, A. ; Thompson, J. ; Davies, G. J. ; Anderson, W. F. Novel Catalytic Mechanism Of Glycoside Hydrolysis Based On The Structure Of An Nad(+)/mn(2+)-Dependent Phospho-Alpha-Glucosidase From Bacillus Subtilis. STRUCTURE 2004, 12, 1619-1629.
Yang, J. K. ; Yoon, H. J. ; Ahn, H. J. ; Lee, B. I. ; Pedelacq, J. D. ; Liong, E. C. ; Berendzen, J. ; Laivenieks, M. ; Vieille, C. ; Zeikus, G. J. ; Vocadlo, D. J. ; Withers, S. G. ; Suh, S. W. Crystal Structure Of Beta-D-Xylosidase From Thermoanaerobacterium Saccharolyticum, A Family 39 Glycoside Hydrolase. JOURNAL OF MOLECULAR BIOLOGY 2004, 335, 155-165.
Yip, V. L. Y. ; Varrot, A. ; Davies, G. J. ; Rajan, S. S. ; Yang, X. J. ; Thompson, J. ; Anderson, W. F. ; Withers, S. G. An Unusual Mechanism Of Glycoside Hydrolysis Involving Redox And Elimination Steps By A Family 4 Beta-Glycosidase From Thermotoga Maritima. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2004, 126, 8354-8355.
Gloster, T. M. ; Williams, S. J. ; Roberts, S. ; Tarling, C. A. ; Wicki, J. ; Withers, S. G. ; Davies, G. J. Atomic Resolution Analyses Of The Binding Of Xylobiose-Derived Deoxynojirimycin And Isofagomine To Xylanase Xyn10A. CHEMICAL COMMUNICATIONS 2004, 1794-1795.
Damager, I. ; Numao, S. ; Chen, H. M. ; BRAYER, G. D. ; Withers, S. G. Synthesis And Characterisation Of Novel Chromogenic Substrates For Human Pancreatic Alpha-Amylase. CARBOHYDRATE RESEARCH 2004, 339, 1727-1737.
Yip, V. L. Y. ; Withers, S. G. Nature's Many Mechanisms For The Degradation Of Oligosaccharides. ORGANIC & BIOMOLECULAR CHEMISTRY 2004, 2, 2707-2713.
Lairson, L. L. ; Chiu, C. P. C. ; Ly, H. D. ; He, S. M. ; WAKARCHUK, W. W. ; Strynadka, N. C. J. ; Withers, S. G. Intermediate Trapping On A Mutant Retaining Alpha-Galactosyltransferase Identifies An Unexpected Aspartate Residue. JOURNAL OF BIOLOGICAL CHEMISTRY 2004, 279, 28339-28344.
Wrodnigg, T. M. ; Diness, F. ; Gruber, C. ; Hausler, H. ; Lundt, I. ; RUPITZ, K. ; Steiner, A. J. ; Stutz, A. E. ; Tarling, C. A. ; Withers, S. G. ; Wolfler, H. Probing The Aglycon Binding Site Of A Beta-Glucosidase: A Collection Of C-1-Modified 2,5-Dideoxy-2,5-Imino-D-Mannitol Derivatives And Their Structure-Activity Relationships As Competitive Inhibitors. BIOORGANIC & MEDICINAL CHEMISTRY 2004, 12, 3485-3495.
Amaya, M. F. ; Watts, A. G. ; Damager, I. ; Wehenkel, A. ; Nguyen, T. ; Buschiazzo, A. ; Paris, G. ; Frasch, A. C. ; Withers, S. G. ; Alzari, P. M. Structural Insights Into The Catalytic Mechanism Of Trypanosoma Cruzi Trans-Sialidase. STRUCTURE 2004, 12, 775-784.
Tropak, M. B. ; Reid, S. P. ; Guiral, M. ; Withers, S. G. ; Mahuran, D. Pharmacological Enhancement Of Beta-Hexosaminidase Activity In Fibroblasts From Adult Tay-Sachs And Sandhoff Patients. JOURNAL OF BIOLOGICAL CHEMISTRY 2004, 279, 13478-13487.
Sulzenbacher, G. ; Bignon, C. ; Nishimura, T. ; Tarling, C. A. ; Withers, S. G. ; Henrissat, B. ; Bourne, Y. Crystal Structure Of Thermotoga Maritima Alpha-L-Fucosidase - Insights Into The Catalytic Mechanism And The Molecular Basis For Fucosidosis. JOURNAL OF BIOLOGICAL CHEMISTRY 2004, 279, 13119-13128.
Gloster, T. M. ; Macdonald, J. M. ; Tarling, C. A. ; Stick, R. V. ; Withers, S. G. ; Davies, G. J. Structural, Thermodynamic, And Kinetic Analyses Of Tetrahydrooxazine-Derived Inhibitors Bound To Beta-Glucosidases. JOURNAL OF BIOLOGICAL CHEMISTRY 2004, 279, 49236-49242.
Jahn, M. ; Chen, H. M. ; Mullegger, J. ; Marles, J. ; WARREN, R. A. J. ; Withers, S. G. Thioglycosynthases: Double Mutant Glycosidases That Serve As Scaffolds For Thioglycoside Synthesis. CHEMICAL COMMUNICATIONS 2004, 274-275.

2003

Varrot, A. ; Tarling, C. A. ; Macdonald, J. M. ; Stick, R. V. ; Zechel, D. L. ; Withers, S. G. ; Davies, G. J. Direct Observation Of The Protonation State Of An Imino Sugar Glycosidase Inhibitor Upon Binding. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2003, 125, 7496-7497.
Watts, A. G. ; Damager, I. ; Amaya, M. L. ; Buschiazzo, A. ; Alzari, P. ; Frasch, A. C. ; Withers, S. G. Trypanosoma Cruzi Trans-Sialidase Operates Through A Covalent Sialyl-Enzyme Intermediate: Tyrosine Is The Catalytic Nucleophile. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2003, 125, 7532-7533.
Zechel, D. L. ; Reid, S. P. ; Stoll, D. ; Nashiru, O. ; WARREN, R. A. J. ; Withers, S. G. Mechanism, Mutagenesis, And Chemical Rescue Of Beta-Mannosidase From Cellulomonas Fimi. BIOCHEMISTRY 2003, 42, 7195-7204.
Poon, D. K. Y. ; Webster, P. ; Withers, S. G. ; McIntosh, L. P. Characterizing The Ph-Dependent Stability And Catalytic Mechanism Of The Family 11 Xylanase From The Alkalophilic Bacillus Agaradhaerens. CARBOHYDRATE RESEARCH 2003, 338, 415-421.
Jahn, M. ; Marles, J. ; WARREN, R. A. J. ; Withers, S. G. Thioglycoligases: Mutant Glycosidases For Thioglycoside Synthesis. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2003, 42, 352+.
Gloster, T. ; Williams, S. J. ; Tarling, C. A. ; Roberts, S. ; Dupont, C. ; Jodoin, P. ; Shareck, F. ; Withers, S. G. ; Davies, G. J. A Xylobiose-Derived Isofagomine Lactam Glycosidase Inhibitor Binds As Its Amide Tautomer. CHEMICAL COMMUNICATIONS 2003, 944-945.
Jahn, M. ; Stoll, D. ; WARREN, R. A. J. ; Szabo, L. ; Singh, P. ; Gilbert, H. J. ; Ducros, V. M. A. ; Davies, G. J. ; Withers, S. G. Expansion Of The Glycosynthase Repertoire To Produce Defined Manno-Oligosaccharides. CHEMICAL COMMUNICATIONS 2003, 1327-1329.
Tarling, C. A. ; He, S. M. ; Sulzenbacher, G. ; Bignon, C. ; Bourne, Y. ; Henrissat, B. ; Withers, S. G. Identification Of The Catalytic Nucleophile Of The Family 29 Alpha-L-Fucosidase From Thermotoga Maritima Through Trapping Of A Covalent Glycosyl-Enzyme Intermediate And Mutagenesis. JOURNAL OF BIOLOGICAL CHEMISTRY 2003, 278, 47394-47399.
Numao, S. ; Kuntz, D. A. ; Withers, S. G. ; ROSE, D. R. Insights Into The Mechanism Of Drosophila Melanogaster Golgi Alpha-Mannosidase Ii Through The Structural Analysis Of Covalent Reaction Intermediates. JOURNAL OF BIOLOGICAL CHEMISTRY 2003, 278, 48074-48083.
Lee, S. S. ; Yu, S. ; Withers, S. G. Detailed Dissection Of A New Mechanism For Glycoside Cleavage: Alpha-1,4-Glucan Lyase. BIOCHEMISTRY 2003, 42, 13081-13090.
Jahn, M. ; Withers, S. G. New Approaches To Enzymatic Oligosaccharide Synthesis: Glycosynthases And Thioglycoligases. BIOCATALYSIS AND BIOTRANSFORMATION 2003, 21, 159-166.
Nieman, C. E. ; Wong, A. W. ; He, S. M. ; Clarke, L. ; Hopwood, J. J. ; Withers, S. G. Family 39 Alpha-L-Iduronidases And Beta-D-Xylosidases React Through Similar Glycosyl-Enzyme Intermediates: Identification Of The Human Iduronidase Nucleophile. BIOCHEMISTRY 2003, 42, 8054-8065.
Ducros, V. M. A. ; Tarling, C. A. ; Zechel, D. L. ; Brzozowski, A. M. ; Frandsen, T. P. ; von Ossowski, I. ; Schulein, M. ; Withers, S. G. ; Davies, G. J. Anatomy Of Glycosynthesis: Structure And Kinetics Of The Humicola Insolens Cel7B E197A And E197S Glycosynthase Mutants. CHEMISTRY & BIOLOGY 2003, 10, 619-628.

2002

Lee, S. S. ; Yu, S. K. ; Withers, S. G. Alpha-1,4-Glucan Lyase Performs A Trans-Elimination Via A Nucleophilic Displacement Followed By A Syn-Elimination. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2002, 124, 4948-4949.
Ly, H. D. ; Lougheed, B. ; WAKARCHUK, W. W. ; Withers, S. G. Mechanistic Studies Of A Retaining Alpha-Galactosyltransferase From Neisseria Meningitidis. BIOCHEMISTRY 2002, 41, 5075-5085.
Rydberg, E. H. ; Li, C. M. ; Maurus, R. ; Overall, C. M. ; BRAYER, G. D. ; Withers, S. G. Mechanistic Analyses Of Catalysis In Human Pancreatic Alpha-Amylase: Detailed Kinetic And Structural Studies Of Mutants Of Three Conserved Carboxylic Acids. BIOCHEMISTRY 2002, 41, 4492-4502.
Hausler, H. ; RUPITZ, K. ; Stutz, A. E. ; Withers, S. G. N-Alkylated Derivatives Of 1,5-Dideoxy-1,5-Iminoxylitol As Beta-Xylosidase And Beta-Glucosidase Inhibitors. MONATSHEFTE FUR CHEMIE 2002, 133, 555-560.
Agnihotri, G. ; He, S. M. ; Hong, L. ; Dakoji, S. ; Withers, S. G. ; Liu, H. W. A Revised Mechanism For The Inactivation Of Bovine Liver Enoyl-Coa Hydratase By (Methylenecyclopropyl)Formyl-Coa Based On Unexpected Results With The C114A Mutant. BIOCHEMISTRY 2002, 41, 1843-1852.
Numao, S. ; Maurus, R. ; Sidhu, G. ; Wang, Y. ; Overall, C. M. ; BRAYER, G. D. ; Withers, S. G. Probing The Role Of The Chloride Ion In The Mechanism Of Human Pancreatic Alpha-Amylase. BIOCHEMISTRY 2002, 41, 215-225.
Ducros, V. M. A. ; Zechel, D. L. ; Murshudov, G. N. ; Gilbert, H. J. ; Szabo, L. ; Stoll, D. ; Withers, S. G. ; Davies, G. J. Substrate Distortion By A Beta-Mannanase: Snapshots Of The Michaelis And Covalent-Intermediate Complexes Suggest A B-2,b-5 Conformation For The Transition State. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2002, 41, 2824+.
Withers, S. G. ; WAKARCHUK, W. W. ; Strynadka, N. C. J. One Step Closer To A Sweet Conclusion. CHEMISTRY & BIOLOGY 2002, 9, 1270-1273.
Williams, S. J. ; Withers, S. G. Glycosynthases: Mutant Glycosidases For Glycoside Synthesis. AUSTRALIAN JOURNAL OF CHEMISTRY 2002, 55, 3-12.
Williams, S. J. ; Mark, B. L. ; Vocadlo, D. J. ; James, M. N. G. ; Withers, S. G. Aspartate 313 In The Streptomyces Plicatus Hexosaminidase Plays A Critical Role In Substrate-Assisted Catalysis By Orienting The 2-Acetamido Group And Stabilizing The Transition State. JOURNAL OF BIOLOGICAL CHEMISTRY 2002, 277, 40055-40065.
Macdonald, J. M. ; Stick, R. V. ; Tilbrook, D. M. G. ; Withers, S. G. Synthesis With Glycosynthases: Cello-Oligomers Of Isofagomine And A Tetrahydrooxazine As Cellulase Inhibitors. AUSTRALIAN JOURNAL OF CHEMISTRY 2002, 55, 747-752.
Jakeman, D. L. ; Withers, S. G. Engineering Glycosidases For Constructive Purposes. In CARBOHYDRATE BIOENGINEERING: INTERDISCIPLINARY APPROACHES; Teeri, T. T. ; Svensson, B. ; Gilbert, H. J. ; Feizi, T. ; CARBOHYDRATE BIOENGINEERING: INTERDISCIPLINARY APPROACHES; Bo Rydin Fdn Sci Res; Swedish Natl Sci Res Council; Swedish Council Forestry & Agr Res; Appl Biosyst; Genencor Int Inc; Neose Technologies Inc; Micromass AB; Novozymes; Eka Chm; Ezaki Glio Co Ltd; Amano Enzyme Inc; Nihon Shokuhin Kako Co Ltd; Nagase Bioch, 2002; pp. 3-8.
Rye, C. S. ; Withers, S. G. Elucidation Of The Mechanism Of Polysaccharide Cleavage By Chondroitin Ac Lyase From Flavobacterium Heparinum. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2002, 124, 9756-9767.
Mosi, R. M. ; Withers, S. G. Trapping Of Alpha-Glycosidase Intermediates. In ENZYME KINETICS AND MECHANISM, PT F: DETECTION AND CHARACTERIZATION OF ENZYME REACTION INTERMEDIATES; ENZYME KINETICS AND MECHANISM, PT F: DETECTION AND CHARACTERIZATION OF ENZYME REACTION INTERMEDIATES; 2002; Vol. 354, pp. 64-84.
Vocadlo, D. J. ; Wicki, J. ; RUPITZ, K. ; Withers, S. G. Mechanism Of Thermoanaerobacterium Saccharolyticum Ss-Xylosidase: Kinetic Studies. BIOCHEMISTRY 2002, 41, 9727-9735.
Wicki, J. ; ROSE, D. R. ; Withers, S. G. Trapping Covalent Intermediates On Beta-Glycosidases. In ENZYME KINETICS AND MECHANISM, PT F: DETECTION AND CHARACTERIZATION OF ENZYME REACTION INTERMEDIATES; Purich, D. L. ; ENZYME KINETICS AND MECHANISM, PT F: DETECTION AND CHARACTERIZATION OF ENZYME REACTION INTERMEDIATES; 2002; Vol. 354, pp. 84-105.
Jakeman, D. L. ; Withers, S. G. Glycosynthases: New Tools For Oligosaccharide Synthesis. TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY 2002, 14, 13-25.
Jakeman, D. L. ; Withers, S. G. On Expanding The Repertoire Of Glycosynthases: Mutant Beta-Galactosidases Forming Beta-(1,6)-Linkages. CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE 2002, 80, 866-870.
Rye, C. S. ; Withers, S. G. Synthesis And Evaluation Of Potential Inhibitors Of Chondroitin Ac Lyase From Flavobacterium Heparinum. JOURNAL OF ORGANIC CHEMISTRY 2002, 67, 4505-4512.
Tolborg, J. F. ; Petersen, L. ; Jensen, K. J. ; Mayer, C. ; Jakeman, D. L. ; WARREN, R. A. J. ; Withers, S. G. Solid-Phase Oligosaccharide And Glycopeptide Synthesis Using Glycosynthases. JOURNAL OF ORGANIC CHEMISTRY 2002, 67, 4143-4149.

2001

Mark, B. L. ; Vocadlo, D. J. ; Knapp, S. ; Triggs-Raine, B. L. ; Withers, S. G. ; James, M. N. G. Crystallographic Evidence For Substrate-Assisted Catalysis In A Bacterial Beta-Hexosaminidase. JOURNAL OF BIOLOGICAL CHEMISTRY 2001, 276, 10330-10337.
Hou, Y. M. ; Vocadlo, D. J. ; Leung, A. ; Withers, S. G. ; Mahuran, D. Characterization Of The Glu And Asp Residues In The Active Site Of Human Beta-Hexosaminidase B. BIOCHEMISTRY 2001, 40, 2201-2209.
Persson, K. ; Ly, H. D. ; Dieckelmann, M. ; WAKARCHUK, W. W. ; Withers, S. G. ; Strynadka, N. C. J. Crystal Structure Of The Retaining Galactosyltransferase Lgtc From Neisseria Meningitidis In Complex With Donor And Acceptor Sugar Analogs. NATURE STRUCTURAL BIOLOGY 2001, 8, 166-175.
Nashiru, O. ; Zechel, D. L. ; Stoll, D. ; Mohammadzadeh, T. ; WARREN, R. A. J. ; Withers, S. G. Beta-Mannosynthase: Synthesis Of Beta-Mannosides With A Mutant Beta-Mannosidase. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2001, 40, 417-420.
Brun, E. ; Brumer, H. ; MacKenzie, L. F. ; Withers, S. G. ; McIntosh, L. P. Letter To The Editor: Assignment Of Selectively C-13-Labeled Cellopentaose Synthesized Using An Engineered Glycosynthase. JOURNAL OF BIOMOLECULAR NMR 2001, 21, 67-68.
Joshi, M. D. ; Sidhu, G. ; Nielsen, J. E. ; BRAYER, G. D. ; Withers, S. G. ; McIntosh, L. P. Dissecting The Electrostatic Interactions And Ph-Dependent Activity Of A Family 11 Glycosidase. BIOCHEMISTRY 2001, 40, 10115-10139.
Vocadlo, D. J. ; Davies, G. J. ; Laine, R. ; Withers, S. G. Catalysis By Hen Egg-White Lysozyme Proceeds Via A Covalent Intermediate. NATURE 2001, 412, 835-838.
Blanchard, J. E. ; Gal, L. ; He, S. M. ; Foisy, J. ; WARREN, R. A. J. ; Withers, S. G. The Identification Of The Catalytic Nucleophiles Of Two Beta-Galactosidases From Glycoside Hydrolase Family 35. CARBOHYDRATE RESEARCH 2001, 333, 7-17.
Hermetter, A. ; Scholze, H. ; Stutz, A. E. ; Withers, S. G. ; Wrodnigg, T. M. Powerful Probes For Glycosidases: Novel, Fluorescently Tagged Glycosidase Inhibitors. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 2001, 11, 1339-1342.
Zechel, D. L. ; Reid, S. P. ; Nashiru, O. ; Mayer, C. ; Stoll, D. ; Jakeman, D. L. ; Warren, P. A. J. ; Withers, S. G. Enzymatic Synthesis Of Carbon-Fluorine Bonds. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2001, 123, 4350-4351.
Wong, A. W. ; He, S. M. ; Withers, S. G. Synthesis Of 5-Fluoro-Beta-D-Glucopyranosyluronic Acid Fluoride And Its Evaluation As A Mechanistic Probe Of Escherichia Coli Beta-Glucuronidase. CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE 2001, 79, 510-518.
Mayer, C. ; Jakeman, D. L. ; Mah, M. ; Karjala, G. ; Gal, L. ; WARREN, R. A. J. ; Withers, S. G. Directed Evolution Of New Glycosynthases From Agrobacterium Beta-Glucosidase: A General Screen To Detect Enzymes For Oligosaccharide Synthesis. CHEMISTRY & BIOLOGY 2001, 8, 437-443.
Juers, D. H. ; Heightman, T. D. ; VASELLA, A. ; MCCARTER, J. D. ; Mackenzie, L. ; Withers, S. G. ; Matthews, B. W. A Structural View Of The Action Of Escherichia Coli (Lacz) Beta-Galactosidase. BIOCHEMISTRY 2001, 40, 14781-14794.
Withers, S. G. Mechanisms Of Glycosyl Transferases And Hydrolases. CARBOHYDRATE POLYMERS 2001, 44, 325-337.
Zechel, D. L. ; Withers, S. G. Dissection Of Nucleophilic And Acid-Base Catalysis In Glycosidases. CURRENT OPINION IN CHEMICAL BIOLOGY 2001, 5, 643-649.
Wong, A. W. ; ADAM, M. J. ; Withers, S. G. Synthesis Of 2,6-Dideoxy-2-Fluoro-6-[F-18]-Fluoro-Beta-D-Glucopyranosyl Fluoride (2,6Fgf) As A Potential Imaging Probe For Glucocerebrosidase. JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS 2001, 44, 385-394.
Mark, B. L. ; Vocadlo, D. J. ; Zhao, D. L. ; Knapp, S. ; Withers, S. G. ; James, M. N. G. Biochemical And Structural Assessment Of The 1-N-Azasugar Galnac-Isofagomine As A Potent Family 20 Beta-N-Acetylhexosaminidase Inhibitor. JOURNAL OF BIOLOGICAL CHEMISTRY 2001, 276, 42131-42137.

2000

Connelly, G. P. ; Withers, S. G. ; McIntosh, L. P. Analysis Of The Dynamic Properties Of Bacillus Circulans Xylanase Upon Formation Of A Covalent Glycosyl-Enzyme Intermediate. PROTEIN SCIENCE 2000, 9, 512-524.
Namchuk, M. N. ; MCCARTER, J. D. ; Becalski, A. ; Andrews, T. ; Withers, S. G. The Role Of Sugar Substituents In Glycoside Hydrolysis. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2000, 122, 1270-1277.
Dan, S. ; Marton, I. ; Dekel, M. ; Bravdo, B. A. ; He, S. M. ; Withers, S. G. ; Shoseyov, O. Cloning, Expression, Characterization, And Nucleophile Identification Of Family 3, Aspergillus Niger Beta-Glucosidase. JOURNAL OF BIOLOGICAL CHEMISTRY 2000, 275, 4973-4980.
Mayer, C. ; Zechel, D. L. ; Reid, S. P. ; WARREN, R. A. J. ; Withers, S. G. The E358S Mutant Of Agrobacterium Sp Beta-Glucosidase Is A Greatly Improved Glycosynthase. FEBS LETTERS 2000, 466, 40-44.
Arribas, J. C. D. ; Herrero, A. G. ; Martin-Lomas, M. ; Canada, F. J. ; He, S. M. ; Withers, S. G. Differential Mechanism-Based Labeling And Unequivocal Activity Assignment Of The Two Active Sites Of Intestinal Lactase/phlorizin Hydrolase. EUROPEAN JOURNAL OF BIOCHEMISTRY 2000, 267, 6996-7005.
Numao, S. ; He, S. M. ; Evjen, G. ; Howard, S. ; Tollersrud, O. K. ; Withers, S. G. Identification Of Asp197 As The Catalytic Nucleophile In The Family 38 Alpha-Mannosidase From Bovine Kidney Lysosomes. FEBS LETTERS 2000, 484, 175-178.
Stoll, D. ; He, S. M. ; Withers, S. G. ; WARREN, R. A. J. Identification Of Glu-519 As The Catalytic Nucleophile In Beta-Mannosidase 2A From Cellulomonas Fimi. BIOCHEMICAL JOURNAL 2000, 351, 833-838.
Rye, C. S. ; Withers, S. G. Glycosidase Mechanisms. CURRENT OPINION IN CHEMICAL BIOLOGY 2000, 4, 573-580.
Notenboom, V. ; Williams, S. J. ; HOOS, R. ; Withers, S. G. ; ROSE, D. R. Detailed Structural Analysis Of Glycosidase/inhibitor Interactions: Complexes Of Cex From Cellulomonas Fimi With Xylobiose-Derived Aza-Sugars. BIOCHEMISTRY 2000, 39, 11553-11563.
Hart, D. O. ; He, S. M. ; Chany, C. J. ; Withers, S. G. ; Sims, P. F. G. ; Sinnott, M. L. ; Brumer, H. Identification Of Asp-130 As The Catalytic Nucleophile In The Main Alpha-Galactosidase From Phanerochaete Chrysosporium, A Family 27 Glycosyl Hydrolase. BIOCHEMISTRY 2000, 39, 9826-9836.
Williams, S. J. ; Withers, S. G. Glycosyl Fluorides In Enzymatic Reactions. CARBOHYDRATE RESEARCH 2000, 327, 27-46.
Joshi, M. D. ; Sidhu, G. ; Pot, I. ; BRAYER, G. D. ; Withers, S. G. ; McIntosh, L. P. Hydrogen Bonding And Catalysis: A Novel Explanation For How A Single Amino Acid Substitution Can Change The Ph Optimum Of A Glycosidase. JOURNAL OF MOLECULAR BIOLOGY 2000, 299, 255-279.
Zechel, D. L. ; Withers, S. G. Glycosidase Mechanisms: Anatomy Of A Finely Tuned Catalyst. ACCOUNTS OF CHEMICAL RESEARCH 2000, 33, 11-18.
Williams, S. J. ; Notenboom, V. ; Wicki, J. ; ROSE, D. R. ; Withers, S. G. A New, Simple, High-Affinity Glycosidase Inhibitor: Analysis Of Binding Through X-Ray Crystallography, Mutagenesis, And Kinetic Analysis. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2000, 122, 4229-4230.
Fairweather, J. K. ; Stick, R. V. ; Withers, S. G. A `glycosynthase'-Assisted Synthesis Of Some Epoxyalkyl Beta-C-Oligosaccharides. AUSTRALIAN JOURNAL OF CHEMISTRY 2000, 53, 913-916.
BRAYER, G. D. ; Sidhu, G. ; Maurus, R. ; Rydberg, E. H. ; BRAUN, C. ; WANG, Y. L. ; Nguyen, N. T. ; Overall, C. H. ; Withers, S. G. Subsite Mapping Of The Human Pancreatic Alpha-Amylase Active Site Through Structural, Kinetic, And Mutagenesis Techniques. BIOCHEMISTRY 2000, 39, 4778-4791.
Wrodnigg, T. M. ; Gaderbauer, W. ; Greimel, P. ; Hausler, H. ; Sprenger, F. K. ; Stutz, A. E. ; Virgona, C. ; Withers, S. G. Biologically Active 1-Aminodeoxy And 1-O-Alkyl Derivatives Of The Powerful D-Glucosidase Inhibitor 2,5-Dideoxy-2,5-Imino-D-Mannitol. JOURNAL OF CARBOHYDRATE CHEMISTRY 2000, 19, 975-990.
Williams, S. J. ; HOOS, R. ; Withers, S. G. Nanomolar Versus Millimolar Inhibition By Xylobiose-Derived Azasugars: Significant Differences Between Two Structurally Distinct Xylanases. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2000, 122, 2223-2235.

1999

Lougheed, B. ; Ly, H. D. ; WAKARCHUK, W. W. ; Withers, S. G. Glycosyl Fluorides Can Function As Substrates For Nucleotide Phosphosugar-Dependent Glycosyltransferases. JOURNAL OF BIOLOGICAL CHEMISTRY 1999, 274, 37717-37722.
Uitdehaag, J. C. M. ; Mosi, R. ; Kalk, K. H. ; van der Veen, B. A. ; Dijkhuizen, L. ; Withers, S. G. ; Dijkstra, B. W. X-Ray Structures Along The Reaction Pathway Of Cyclodextrin Glycosyltransferase Elucidate Catalysis In The Alpha-Amylase Family. NATURE STRUCTURAL BIOLOGY 1999, 6, 432-436.
Sidhu, G. ; Withers, S. G. ; Nguyen, N. T. ; McIntosh, L. P. ; ZISER, L. ; BRAYER, G. D. Sugar Ring Distortion In The Glycosyl-Enzyme Intermediate Of A Family G/11 Xylanase. BIOCHEMISTRY 1999, 38, 5346-5354.
Sulzenbacher, G. ; MacKenzie, L. F. ; Wilson, K. S. ; Withers, S. G. ; Dupont, C. ; Davies, G. J. The Crystal Structure Of A 2-Fluorocellotriosyl Complex Of The Streptomyces Lividans Endoglucanase Celb2 At 1.2 Angstrom Resolution. BIOCHEMISTRY 1999, 38, 4826-4833.
Johnson, P. E. ; Brun, E. ; MacKenzie, L. F. ; Withers, S. G. ; McIntosh, L. P. The Cellulose-Binding Domains From Cellulomonas Fimi Beta-1,4-Glucanase Cenc Bind Nitroxide Spin-Labeled Cellooligosaccharides In Multiple Orientations. JOURNAL OF MOLECULAR BIOLOGY 1999, 287, 609-625.
Rydberg, E. H. ; Sidhu, G. ; Vo, H. C. ; Hewitt, J. ; Cote, H. C. F. ; WANG, Y. L. ; Numao, S. ; MacGillivray, R. T. A. ; Overall, C. M. ; BRAYER, G. D. ; Withers, S. G. Cloning, Mutagenesis, And Structural Analysis Of Human Pancreatic Alpha-Amylase Expressed In Pichia Pastoris. PROTEIN SCIENCE 1999, 8, 635-643.
Ly, H. D. ; Withers, S. G. Mutagenesis Of Glycosidases. ANNUAL REVIEW OF BIOCHEMISTRY 1999, 68, 487-522.
Withers, S. G. 1998 Hoffmann La Roche Award Lecture - Understanding And Exploiting Glycosidases. CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE 1999, 77, 1-11.

1998

Davies, G. J. ; Mackenzie, L. ; Varrot, A. ; Dauter, M. ; Brzozowski, A. M. ; Schulein, M. ; Withers, S. G. Snapshots Along An Enzymatic Reaction Coordinate: Analysis Of A Retaining Beta-Glycoside Hydrolase. BIOCHEMISTRY 1998, 37, 11707-11713.
MacKenzie, L. F. ; WANG, Q. P. ; WARREN, R. A. J. ; Withers, S. G. Glycosynthases: Mutant Glycosidases For Oligosaccharide Synthesis. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1998, 120, 5583-5584.
Zechel, D. L. ; Konermann, L. ; Withers, S. G. ; Douglas, D. J. Pre-Steady State Kinetic Analysis Of An Enzymatic Reaction Monitored By Time-Resolved Electrospray Ionization Mass Spectrometry. BIOCHEMISTRY 1998, 37, 7664-7669.
Birsan, C. ; Johnson, P. ; Joshi, M. ; MacLeod, A. ; McIntosh, L. ; Monem, V. ; Nitz, M. ; ROSE, D. R. ; TULL, D. ; Wakarchuck, W. W. ; WANG, Q. ; WARREN, R. A. J. ; White, A. ; Withers, S. G. Mechanisms Of Cellulases And Xylanases. BIOCHEMICAL SOCIETY TRANSACTIONS 1998, 26, 156-160.
Notenboom, V. ; Birsan, C. ; WARREN, R. A. J. ; Withers, S. G. ; ROSE, D. R. Exploring The Cellulose/xylan Specificity Of The Beta-1,4-Glycanase Cex From Cellulomonas Fimi Through Crystallography And Mutation. BIOCHEMISTRY 1998, 37, 4751-4758.
Ferro, V. ; Weiler, L. ; Withers, S. G. ; Ziltener, H. N-Glycosyl Phosphonamidates: Potential Transition-State Analogue Inhibitors Of Glycopeptidases. CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE 1998, 76, 313-318.
Ferro, V. ; Weiler, L. ; Withers, S. G. Convergent Synthesis Of A Fluorescence-Quenched Glycopeptide As A Potential Substrate For Peptide: N-Glycosidases. CARBOHYDRATE RESEARCH 1998, 306, 531-538.
Wong, A. W. ; He, S. M. ; Grubb, J. H. ; Sly, W. S. ; Withers, S. G. Identification Of Glu-540 As The Catalytic Nucleophile Of Human Beta-Glucuronidase Using Electrospray Mass Spectrometry. JOURNAL OF BIOLOGICAL CHEMISTRY 1998, 273, 34057-34062.
Howard, S. ; He, S. M. ; Withers, S. G. Identification Of The Active Site Nucleophile In Jack Bean Alpha-Mannosidase Using 5-Fluoro-Beta-L-Guloxyl Fluoride. JOURNAL OF BIOLOGICAL CHEMISTRY 1998, 273, 2067-2072.
Mosi, R. ; Sham, H. ; Uitdehaag, J. C. M. ; Ruiterkamp, R. ; Dijkstra, B. W. ; Withers, S. G. Reassessment Of Acarbose As A Transition State Analogue Inhibitor Of Cyclodextrin Glycosyltransferase. BIOCHEMISTRY 1998, 37, 17192-17198.
Zechel, D. L. ; He, S. M. ; Dupont, C. ; Withers, S. G. Identification Of Glu-120 As The Catalytic Nucleophile In Streptomyces Lividans Endoglucanase Celb. BIOCHEMICAL JOURNAL 1998, 336, 139-145.
MacKenzie, L. F. ; Sulzenbacher, G. ; Divne, C. ; Jones, T. A. ; Woldike, H. F. ; Schulein, M. ; Withers, S. G. ; Davies, G. J. Crystal Structure Of The Family 7 Endoglucanase I (Cel7B) From Humicola Insolens At 2.2 Angstrom Resolution And Identification Of The Catalytic Nucleophile By Trapping Of The Covalent Glycosyl-Enzyme Intermediate. BIOCHEMICAL JOURNAL 1998, 335, 409-416.
Vocadlo, D. J. ; MacKenzie, L. F. ; He, S. M. ; Zeikus, G. J. ; Withers, S. G. Identification Of Glu-277 As The Catalytic Nucleophile Of Thermoanaerobacterium Saccharolyticum Beta-Xylosidase Using Electrospray Ms. BIOCHEMICAL JOURNAL 1998, 335, 449-455.
Howard, S. ; Withers, S. G. Bromoketone C-Glycosides, A New Class Of Beta-Glucanase Inactivators. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1998, 120, 10326-10331.
Notenboom, V. ; Birsan, C. ; Nitz, M. ; ROSE, D. R. ; WARREN, R. A. J. ; Withers, S. G. Insights Into Transition State Stabilization Of The Beta-1,4-Glycosidase Cex By Covalent Intermediate Accumulation In Active Site Mutants. NATURE STRUCTURAL BIOLOGY 1998, 5, 812-818.

1997

Lawson, S. L. ; WAKARCHUK, W. W. ; Withers, S. G. Positioning The Acid/base Catalyst In A Glycosidase: Studies With Bacillus Circulans Xylanase. BIOCHEMISTRY 1997, 36, 2257-2265.
MCCARTER, J. D. ; Yeung, W. ; Chow, J. ; DOLPHIN, D. ; Withers, S. G. Design And Synthesis Of 2'-Deoxy-2'-Fluorodisaccharides As Mechanism-Based Glycosidase Inhibitors That Exploit Aglycon Specificity. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1997, 119, 5792-5797.
MacKenzie, L. F. ; Brooke, G. S. ; Cutfield, J. F. ; Sullivan, P. A. ; Withers, S. G. Identification Of Glu-330 As The Catalytic Nucleophile Of Candida Albicans Exo-Beta-(1,3)-Glucanase. JOURNAL OF BIOLOGICAL CHEMISTRY 1997, 272, 3161-3167.
Andersen, S. M. ; Ebner, M. ; Ekhart, C. W. ; Gradnig, G. ; Legler, G. ; Lundt, I. ; Stutz, A. E. ; Withers, S. G. ; Wrodnigg, T. Two Isosteric Fluorinated Derivatives Of The Powerful Glucosidase Inhibitors, 1-Deoxynojirimycin And 2,5-Dideoxy-2,5-Imino-D-Mannitol: Syntheses And Glycosidase-Inhibitory Activities Of 1,2,5-Trideoxy-2-Fluoro-1,5-Imino-D-Glucitol And Of 1,2,5-Trideoxy-1-. CARBOHYDRATE RESEARCH 1997, 301, 155-166.
MCCARTER, J. D. ; Burgoyne, D. L. ; MIAO, S. C. ; Zhang, S. Q. ; Callahan, J. W. ; Withers, S. G. Identification Of Glu-268 As The Catalytic Nucleophile Of Human Lysosomal Beta-Galactosidase Precursor By Mass Spectrometry. JOURNAL OF BIOLOGICAL CHEMISTRY 1997, 272, 396-400.
MacKenzie, L. F. ; Davies, G. J. ; Schulein, M. ; Withers, S. G. Identification Of The Catalytic Nucleophile Of Endoglucanase I From Fusarium Oxysporum By Mass Spectrometry. BIOCHEMISTRY 1997, 36, 5893-5901.
Knapp, S. ; Kirk, B. A. ; Vocadlo, D. ; Withers, S. G. An Allosamizoline/glucosamine Hybrid Nagase Inhibitor. SYNLETT 1997, 435-&.
HOOS, R. ; VASELLA, A. ; RUPITZ, K. ; Withers, S. G. D-Glyconhydroximolactams Strongly Inhibit Alpha-Glycosidases. CARBOHYDRATE RESEARCH 1997, 298, 291-298.
Prade, H. ; MacKenzie, L. F. ; Withers, S. G. Enzymatic Synthesis Of Disaccharides Using Agrobacterium Sp. Beta-Glucosidase. CARBOHYDRATE RESEARCH 1997, 305, 371-381.
He, S. M. ; Withers, S. G. Assignment Of Sweet Almond Beta-Glucosidase As A Family 1 Glycosidase And Identification Of Its Active Site Nucleophile. JOURNAL OF BIOLOGICAL CHEMISTRY 1997, 272, 24864-24867.
Harvey, T. C. ; Simiand, C. ; Weiler, L. ; Withers, S. G. Synthesis Of Cyclic Phosphonate Analogs Of Ribose And Arabinose. JOURNAL OF ORGANIC CHEMISTRY 1997, 62, 6722-6725.
Howard, S. ; BRAUN, C. ; McCarter, J. ; Moremen, K. W. ; Liao, Y. F. ; Withers, S. G. Human Lysosomal And Jack Bean Alpha-Mannosidases Are Retaining Glycosidases. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1997, 238, 896-898.
Mosi, R. ; He, S. M. ; Uitdehaag, J. ; Dijkstra, B. W. ; Withers, S. G. Trapping And Characterization Of The Reaction Intermediate In Cyclodextrin Glycosyltransferase By Use Of Activated Substrates And A Mutant Enzyme. BIOCHEMISTRY 1997, 36, 9927-9934.

1996

Withers, S. G. Identification Of Active-Site Residues In Glycosidases. In BIOCHEMICAL AND BIOTECHNOLOGICAL APPLICATIONS OF ELECTROSPRAY IONIZATION MASS SPECTROMETRY; Snyder, A. P. ; BIOCHEMICAL AND BIOTECHNOLOGICAL APPLICATIONS OF ELECTROSPRAY IONIZATION MASS SPECTROMETRY; Amer Chem Soc, 1996; Vol. 619, pp. 365-380.
NAMCHUK, M. ; BRAUN, C. ; MCCARTER, J. D. ; Withers, S. G. Fluorinated Sugars As Probes Of Glycosidase Mechanisms. In BIOMEDICAL FRONTIERS OF FLUORINE CHEMISTRY; Ojima, I. ; , ; Welch, J. T. ; BIOMEDICAL FRONTIERS OF FLUORINE CHEMISTRY; Amer Chem Soc, Div Fluorine Chem; Amer Chem Soc, Div Medicinal Chem, 1996; Vol. 639, pp. 279-293.
Bray, M. R. ; Creagh, A. L. ; Damude, H. G. ; GILKES, N. R. ; Haynes, C. A. ; Jervis, E. ; KILBURN, D. G. ; MACLEOD, A. M. ; MEINKE, A. ; MILLER, R. C. ; ROSE, D. R. ; Shen, H. ; Tomme, P. ; TULL, D. ; White, A. ; Withers, S. G. ; WARREN, R. A. J. Beta-1,4-Glycanases Of Cellulomonas Fimi: Families, Mechanisms, And Kinetics. In ENZYMES FOR PULP AND PAPER PROCESSING; Jeffries, T. W. ; Viikari, L. ; ENZYMES FOR PULP AND PAPER PROCESSING; Amer Chem Soc, 1996; Vol. 655, pp. 64-84.
Lai, E. C. K. ; Morris, S. A. ; STREET, I. P. ; Withers, S. G. Substituted Glycals As Probes Of Glycosidase Mechanisms. BIOORGANIC & MEDICINAL CHEMISTRY 1996, 4, 1929-1937.
MACLEOD, A. M. ; TULL, D. ; RUPITZ, K. ; WARREN, R. A. J. ; Withers, S. G. Mechanistic Consequences Of Mutation Of Active Site Carboxylates In A Retaining Beta-1,4-Glycanase From Cellulomonas Fini. BIOCHEMISTRY 1996, 35, 13165-13172.
McIntosh, L. P. ; Hand, G. ; Johnson, P. E. ; Joshi, M. D. ; Korner, M. ; Plesniak, L. A. ; ZISER, L. ; WAKARCHUK, W. W. ; Withers, S. G. The Pk(A) Of The General Acid/base Carboxyl Group Of A Glycosidase Cycles During Catalysis: A C-13-Nmr Study Of Bacillus Circuluns Xylanase. BIOCHEMISTRY 1996, 35, 9958-9966.
Lawson, S. L. ; WAKARCHUK, W. W. ; Withers, S. G. Effects Of Both Shortening And Lengthening The Active Site Nucleophile Of Bacillus Circulans Xylanase On Catalytic Activity. BIOCHEMISTRY 1996, 35, 10110-10118.
Knapp, S. ; Vocadlo, D. ; Gao, Z. N. ; Kirk, B. ; Lou, J. P. ; Withers, S. G. Nag-Thiazoline, An N-Acetyl-Beta-Hexosaminidase Inhibitor That Implicates Acetamido Participation. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1996, 118, 6804-6805.
Mitchell, E. P. ; Withers, S. G. ; ERMERT, P. ; Vasella, A. T. ; Garman, E. F. ; Oikonomakos, N. G. ; JOHNSON, L. N. Ternary Complex Crystal Structures Of Glycogen Phosphorylase With The Transition State Analogue Nojirimycin Tetrazole And Phosphate In The T And R States. BIOCHEMISTRY 1996, 35, 7341-7355.
BRAUN, C. ; LINDHORST, T. ; MADSEN, N. B. ; Withers, S. G. Identification Of Asp 549 As The Catalytic Nucleophile Of Glycogen-Debranching Enzyme Via Trapping Of The Glycosyl-Enzyme Intermediate. BIOCHEMISTRY 1996, 35, 5458-5463.
Damude, H. G. ; Ferro, V. ; Withers, S. G. ; WARREN, R. A. J. Substrate Specificity Of Endoglucanase A From Cellulomonas Fimi: Fundamental Differences Between Endoglucanases And Exoglucanases From Family 6. BIOCHEMICAL JOURNAL 1996, 315, 467-472.
White, A. ; TULL, D. ; Johns, K. ; Withers, S. G. ; ROSE, D. R. Crystallographic Observation Of A Covalent Catalytic Intermediate In A Beta-Glycosidase. NATURE STRUCTURAL BIOLOGY 1996, 3, 149-154.
MCCARTER, J. D. ; Withers, S. G. 5-Fluoro Glycosides: A New Class Of Mechanism-Based Inhibitors Of Both Alpha- And Beta-Glucosidases. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1996, 118, 241-242.

1995

Withers, S. G. ; AEBERSOLD, R. Approaches To Labeling And Identification Of Active-Site Residues In Glycosidases. PROTEIN SCIENCE 1995, 4, 361-372.
Damude, H. G. ; Withers, S. G. ; KILBURN, D. G. ; MILLER, R. C. ; WARREN, R. A. J. Site-Directed Mutation Of The Putative Catalytic Residues Of Endoglucanase Cena From Cellulomonas-Fumi. BIOCHEMISTRY 1995, 34, 2220-2224.
MCCARTER, J. D. ; ADAM, M. J. ; Withers, S. G. Syntheses, Radiolabeling, And Kinetic Evaluation Of 2-Deoxy-2-Fluoro-2-Iodo-D-Hexoses For Medical Imaging. CARBOHYDRATE RESEARCH 1995, 266, 273-277.
Withers, S. G. Probing Of Glycosidase Active Sites Through Labeling, Mutagenesis And Kinetic Studies. In CARBOHYDRATE BIOENGINEERING; Petersen, S. B. ; Svensson, B. ; Pedersen, S. ; CARBOHYDRATE BIOENGINEERING; Biosym Technol; Boehringer DAKO; Dansk Metal; European Commiss, DG XII; European Congress Biotechnol 5; Nordic Fund Technol & Ind Dev; Novo Nordisk A S; Nutek; Pharmacia; Pharmacia Biotech; Radiometer; Silicon Graph; SNF, 1995; Vol. 10, pp. 97-111.
GEBLER, J. C. ; TRIMBUR, D. E. ; WARREN, A. J. ; AEBERSOLD, R. ; NAMCHUK, M. ; Withers, S. G. Substrate-Induced Inactivation Of A Crippled Beta-Glucosidase Mutant - Identification Of The Labeled Amino-Acid And Mutagenic Analysis Of Its Role. BIOCHEMISTRY 1995, 34, 14547-14553.
WANG, Q. ; TRIMBUR, D. ; GRAHAM, R. ; WARREN, R. A. J. ; Withers, S. G. Identification Of The Acid/base Catalyst In Agrobacterium-Faecalis Beta-Glucosidase By Kinetic-Analysis Of Mutants. BIOCHEMISTRY 1995, 34, 14554-14562.
WANG, Q. P. ; Withers, S. G. Substrate-Assisted Catalysis In Glycosidases. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1995, 117, 10137-10138.
Withers, S. G. Enzymatic Cleavage Of Glycosides - How Does It Happen. PURE AND APPLIED CHEMISTRY 1995, 67, 1673-1682.
ZISER, L. ; SETYAWATI, I. ; Withers, S. G. Syntheses And Testing Of Substrates And Mechanism-Based Inactivators For Xylanases. CARBOHYDRATE RESEARCH 1995, 274, 137-153.
STAEDTLER, P. ; HOENIG, S. ; FRANK, R. ; Withers, S. G. ; HENGSTENBERG, W. Identification Of The Active-Site Nucleophile In 6-Phospho-Beta-Galactosidase From Staphylococcus-Aureus By Labeling With Synthetic Inhibitors. EUROPEAN JOURNAL OF BIOCHEMISTRY 1995, 232, 658-663.
BRAYER, G. D. ; LUO, Y. G. ; Withers, S. G. The Structure Of Human Pancreatic Alpha-Amylase At 1.8 Angstrom Resolution And Comparisons With Related Enzymes. PROTEIN SCIENCE 1995, 4, 1730-1742.
BRAUN, C. ; Withers, S. G. An Assay For The Transferase-Activity Of Glycogen Debranching Enzyme. CARBOHYDRATE RESEARCH 1995, 271, 113-118.
STREET, I. P. ; Withers, S. G. Probing The Ionization State Of Substrate Alpha-D-Glucopyranosyl Phosphate Bound To Glycogen-Phosphorylase-B. BIOCHEMICAL JOURNAL 1995, 308, 1017-1023.
Knapp, S. ; DONG, Y. H. ; RUPITZ, K. ; Withers, S. G. Beta-Glucosidase Inhibitors Incorporating Pyridinium As A Glucosyl Mimic. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS 1995, 5, 763-766.

1994

MACLEOD, A. M. ; LINDHORST, T. ; Withers, S. G. ; WARREN, R. A. J. The Acid/base Catalyst In The Exoglucanase/xylanase From Cellulomonas-Fimi Is Glutamic-Acid-127 - Evidence From Detailed Kinetic-Studies Of Mutants. BIOCHEMISTRY 1994, 33, 6371-6376.
WANG, Q. P. ; GRAHAM, R. W. ; TRIMBUR, D. ; WARREN, R. A. J. ; Withers, S. G. Changing Enzymatic-Reaction Mechanisms By Mutagenesis - Conversion Of A Retaining Glucosidase To An Inverting Enzyme. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1994, 116, 11594-11595.
WANG, Q. P. ; DECHERT, U. ; JIRIK, F. ; Withers, S. G. Suicide Inactivation Of Human Prostatic Acid-Phosphatase And A Phosphotyrosine Phosphatase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1994, 200, 577-583.
MIAO, S. C. ; MCCARTER, J. D. ; GRACE, M. E. ; GRABOWSKI, G. A. ; AEBERSOLD, R. ; Withers, S. G. Identification Of Glu(340) As The Active-Site Nucleophile In Human Glucocerebrosidase By Use Of Electrospray Tandem Mass-Spectrometry. JOURNAL OF BIOLOGICAL CHEMISTRY 1994, 269, 10975-10978.
ZISER, L. ; Withers, S. G. A Short Synthesis Of Beta-Xylobiosides. CARBOHYDRATE RESEARCH 1994, 265, 9-17.
VASELLA, A. ; ERMERT, P. ; HOOS, R. ; NAUGHTON, A. B. ; RUPITZ, K. ; THIEL, W. ; WEBER, M. ; WEBHER, W. ; Withers, S. G. Synthesis And Evaluation Of New Glycosidase Inhibitors. In COMPLEX CARBOHYDRATES IN DRUG RESEARCH: STRUCTURAL AND FUNCTIONAL ASPECTS; Bock, K. ; Clausen, H. ; COMPLEX CARBOHYDRATES IN DRUG RESEARCH: STRUCTURAL AND FUNCTIONAL ASPECTS; ALFRED BENZON FDN, 1994; Vol. 36, pp. 134-150.
MCCARTER, J. D. ; Withers, S. G. Mechanisms Of Enzymatic Glycoside Hydrolysis. CURRENT OPINION IN STRUCTURAL BIOLOGY 1994, 4, 885-892.
SCHNABELRAUCH, M. ; VASELLA, A. ; Withers, S. G. Synthesis And Evaluation As Irreversible Glycosidase Inhibitors Of Mono(Glycosylthio)Benzoquinones And Oligo(Glycosylthio)Benzoquinones. HELVETICA CHIMICA ACTA 1994, 77, 778-799.
Zhang, S. Q. ; MCCARTER, J. D. ; OKAMURAOHO, Y. ; YAGHI, F. ; HINEK, A. ; Withers, S. G. ; Callahan, J. W. Kinetic Mechanism And Characterization Of Human Beta-Galactosidase Precursor Secreted By Permanently Transfected Chinese-Hamster Ovary Cells. BIOCHEMICAL JOURNAL 1994, 304, 281-288.
White, A. ; Withers, S. G. ; GILKES, N. R. ; ROSE, D. R. Crystal-Structure Of The Catalytic Domain Of The Beta-1,4-Glycanase Cex From Cellulomonas-Fimi. BIOCHEMISTRY 1994, 33, 12546-12552.
Knapp, S. ; PURANDARE, A. ; RUPITZ, K. ; Withers, S. G. A (1-]4)-Trehazoloid Glucosidase Inhibitor With Aglycon Selectivity. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1994, 116, 7461-7462.

1993

BURK, D. ; WANG, Y. L. ; DOMBROSKI, D. ; BERGHUIS, A. M. ; EVANS, S. V. ; LUO, Y. G. ; Withers, S. G. ; BRAYER, G. D. Isolation, Crystallization And Preliminary Diffraction Analyses Of Human Pancreatic Alpha-Amylase. JOURNAL OF MOLECULAR BIOLOGY 1993, 230, 1084-1085.
CHALLONER, R. ; MCDOWELL, C. A. ; STIRTAN, W. ; Withers, S. G. A Solid-State P-31-Nmr Investigation Of The Allosteric Transition In Glycogen Phosphorylase-B. BIOPHYSICAL JOURNAL 1993, 64, 484-491.
TRIMBUR, D. ; WARREN, R. A. J. ; Withers, S. G. A Beta-Glucosidase From An Agrobacterium Sp - Structure And Biochemistry. ACS SYMPOSIUM SERIES 1993, 533, 42-55.
TRIMBUR, D. ; WARREN, R. A. J. ; Withers, S. G. A Beta-Glucosidase From An Agrobacterium Sp - Structure And Biochemistry. In BETA-GLUCOSIDASES: BIOCHEMISTRY AND MOLECULAR BIOLOGY; Esen, A. ; BETA-GLUCOSIDASES: BIOCHEMISTRY AND MOLECULAR BIOLOGY; AMER CHEM SOC, DIV AGR & FOOD CHEM; NATL SCI FDN, BIOCHEM PROGRAM, 1993; Vol. 533, pp. 42-55.
HOOS, R. ; NAUGHTON, A. B. ; THIEL, W. ; VASELLA, A. ; WEBER, W. ; RUPITZ, K. ; Withers, S. G. D-Gluconhydroximo-1,5-Lactam And Related N-Arylcarbamates - Theoretical Calculations, Structure, Synthesis, And Inhibitory Effect On Beta-Glucosidases. HELVETICA CHIMICA ACTA 1993, 76, 2666-2686.
ERMERT, P. ; VASELLA, A. ; WEBER, M. ; RUPITZ, K. ; Withers, S. G. Configurationally Selective Transition-State Analog Inhibitors Of Glycosidases - A Study With Nojiritetrazoles, A New Class Of Glycosidase Inhibitors. CARBOHYDRATE RESEARCH 1993, 250, 113-128.
BLACK, T. S. ; KISS, L. ; TULL, D. ; Withers, S. G. N-Bromoacetyl-Glycopyranosylamines As Affinity Labels For A Beta-Glucosidase And A Cellulase. CARBOHYDRATE RESEARCH 1993, 250, 195-202.
MCCARTER, J. D. ; ADAM, M. J. ; BRAUN, C. ; NAMCHUK, M. ; TULL, D. ; Withers, S. G. Syntheses Of 2-Deoxy-2-Fluoro Monosaccharide And Oligosaccharide Glycosides From Glycals And Evaluation As Glycosidase Inhibitors. CARBOHYDRATE RESEARCH 1993, 249, 77-90.
STIRTAN, W. ; Withers, S. G. Direct F-19 Nmr Titration Of Phosphorylase Molecules Binding To Fluorine-Labeled Glycogen Particles. CARBOHYDRATE RESEARCH 1993, 249, 253-258.
WANG, Q. P. ; TULL, D. ; MEINKE, A. ; GILKES, N. R. ; WARREN, R. A. J. ; AEBERSOLD, R. ; Withers, S. G. Glu280 Is The Nucleophile In The Active-Site Of Clostridium-Thermocellum Celc, A Family-A Endo-Beta-1,4-Glucanase. JOURNAL OF BIOLOGICAL CHEMISTRY 1993, 268, 14096-14102.

1992

MCCARTER, J. D. ; ADAM, M. J. ; Withers, S. G. Synthesis Of 2-Deoxy-2-[F-18]-Fluoro-Beta-Mannosyl [F-18] Fluoride As A Potential Imaging Probe For Glycosidases. JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS 1992, 31, 1005-1009.
BEDARKAR, S. ; GILKES, N. R. ; KILBURN, D. G. ; KWAN, E. ; ROSE, D. R. ; MILLER, R. C. ; WARREN, R. A. J. ; Withers, S. G. Crystallization And Preliminary-X-Ray Diffraction Analysis Of The Catalytic Domain Of Cex, An Exo-Beta-1,4-Glucanase And Beta-1,4-Xylanase From The Bacterium Cellulomonas-Fimi. JOURNAL OF MOLECULAR BIOLOGY 1992, 228, 693-695.
KEMPTON, J. B. ; Withers, S. G. Mechanism Of Agrobacterium Beta-Glucosidase - Kinetic-Studies. BIOCHEMISTRY 1992, 31, 9961-9969.
Withers, S. G. ; RUPITZ, K. ; TRIMBUR, D. ; WARREN, R. A. J. Mechanistic Consequences Of Mutation Of The Active-Site Nucleophile Glu-358 In Agrobacterium Beta-Glucosidase. BIOCHEMISTRY 1992, 31, 9979-9985.
GEBLER, J. ; GILKES, N. R. ; Claeyssens, M. ; WILSON, D. B. ; BEGUIN, P. ; WAKARCHUK, W. W. ; KILBURN, D. G. ; MILLER, R. C. ; WARREN, R. A. J. ; Withers, S. G. Stereoselective Hydrolysis Catalyzed By Related Beta-1,4-Glucanases And Beta-1,4-Xylanases. JOURNAL OF BIOLOGICAL CHEMISTRY 1992, 267, 12559-12561.
GEBLER, J. C. ; AEBERSOLD, R. ; Withers, S. G. Glu-537, Not Glu-461, Is The Nucleophile In The Active-Site Of (Lac-Z) Beta-Galactosidase From Escherichia-Coli. JOURNAL OF BIOLOGICAL CHEMISTRY 1992, 267, 11126-11130.
TRIMBUR, D. E. ; WARREN, R. A. J. ; Withers, S. G. Region-Directed Mutagenesis Of Residues Surrounding The Active-Site Nucleophile In Beta-Glucosidase From Agrobacterium-Faecalis. JOURNAL OF BIOLOGICAL CHEMISTRY 1992, 267, 10248-10251.
PERCIVAL, M. D. ; Withers, S. G. Synthesis And Testing Of Sugar Phosphofluoridates And Cyclic Phosphates As Inhibitors Of Phosphoglucomutase. JOURNAL OF ORGANIC CHEMISTRY 1992, 57, 811-817.

1991

, ; Withers, S. G. ; GOLDSMITH, E. J. ; FLETTERICK, R. J. ; MADSEN, N. B. Structural Basis For The Activation Of Glycogen Phosphorylase-B By Adenosine-Monophosphate. SCIENCE 1991, 254, 1367-1371.
TULL, D. ; Withers, S. G. ; GILKES, N. R. ; KILBURN, D. G. ; WARREN, R. A. J. ; AEBERSOLD, R. Glutamic-Acid 274 Is The Nucleophile In The Active-Site Of A Retaining Exoglucanase From Cellulomonas-Fimi. JOURNAL OF BIOLOGICAL CHEMISTRY 1991, 266, 15621-15625.
Withers, S. G. ; UMEZAWA, K. Cyclophellitol - A Naturally-Occurring Mechanism-Based Inactivator Of Beta-Glucosidases. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1991, 177, 532-537.
LIU, W. ; MADSEN, N. B. ; BRAUN, C. ; Withers, S. G. Reassessment Of The Catalytic Mechanism Of Glycogen Debranching Enzyme. BIOCHEMISTRY 1991, 30, 1419-1424.

1990

MARTIN, J. L. ; JOHNSON, L. N. ; Withers, S. G. Comparison Of The Binding Of Glucose And Glucose 1-Phosphate Derivatives To T-State Glycogen Phosphorylase-B. BIOCHEMISTRY 1990, 29, 10745-10757.
BERVEN, L. A. ; DOLPHIN, D. ; Withers, S. G. The Base-Catalyzed Anomerization Of Dinitrophenyl Glycosides - Evidence For A Novel Reaction-Mechanism. CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE 1990, 68, 1859-1866.
Withers, S. G. ; WARREN, R. A. J. ; STREET, I. P. ; RUPITZ, K. ; KEMPTON, J. B. ; AEBERSOLD, R. Unequivocal Demonstration Of The Involvement Of A Glutamate Residue As A Nucleophile In The Mechanism Of A Retaining Glycosidase. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1990, 112, 5887-5889.
BIRD, P. ; DOLPHIN, D. H. ; Withers, S. G. The Synthesis Of Protected 5-Azido-5-Deoxy-D-Glucononitriles As Precursors Of Glycosidase Inhibitors. CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE 1990, 68, 317-322.

1989

Withers, S. G. ; STREET, I. P. Beta-Glucosidases - Mechanism And Inhibition. In PLANT CELL WALL POLYMERS: BIOGENESIS AND BIODEGRADATION; LEWIS, N. G. ; PAICE, M. G. ; PLANT CELL WALL POLYMERS: BIOGENESIS AND BIODEGRADATION; AMER CHEM SOC, DIV CELLULOSE PAPER & TEXTILE, 1989; Vol. 399, pp. 597-607.
CARON, G. ; Withers, S. G. Conduritol Aziridine - A New Mechanism-Based Glucosidase Inactivator. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1989, 163, 495-499.
Withers, S. G. ; PERCIVAL, M. D. ; STREET, I. P. The Synthesis And Hydrolysis Of A Series Of Deoxyfluoro-Alpha-D-Glucopyranosyl And Deoxyfluoro-Alpha-D-Glucopyranosyl Phosphates. CARBOHYDRATE RESEARCH 1989, 187, 43-66.
STREET, I. P. ; RUPITZ, K. ; Withers, S. G. Fluorinated And Deoxygenated Substrates As Probes Of Transition-State Structure In Glycogen-Phosphorylase. BIOCHEMISTRY 1989, 28, 1581-1587.
Withers, S. G. ; STREET, I. P. Beta-Glucosidases - Mechanism And Inhibition. ACS SYMPOSIUM SERIES 1989, 399, 597-607.

1988

Withers, S. G. ; PERCIVAL, M. D. ; STREET, I. P. Fluorinated Carbohydrates As Probes Of Mechanism In Phosphoglucomutase And Beta-Glucosidases. BIOCHEMISTRY 1988, 27, 3078.
PERCIVAL, M. D. ; Withers, S. G. Investigation Of Enzyme-Substrate Complexes Of Phosphoglucomutase By F-19 Nmr. BIOCHEMISTRY 1988, 27, 3106.
STREET, I. P. ; Withers, S. G. Mechanism-Based Glycosidase Inhibitors. BIOCHEMISTRY 1988, 27, 3106.
HOLME, K. R. ; HALL, L. D. ; ARMSTRONG, C. R. ; Withers, S. G. Synthesis And Evaluation Of Chitosan-Based, Affinity-Chromatography Materials. CARBOHYDRATE RESEARCH 1988, 173, 285-291.
Withers, S. G. ; STREET, I. P. ; PERCIVAL, M. D. Fluorinated Carbohydrates As Probes Of Enzyme Specificity And Mechanism. ACS SYMPOSIUM SERIES 1988, 374, 59-77.
Withers, S. G. ; STREET, I. P. Identification Of A Covalent Alpha-D-Glucopyranosyl Enzyme Intermediate Formed On A Beta-Glucosidase. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1988, 110, 8551-8553.
PERCIVAL, M. D. ; Withers, S. G. Applications Of Enzymes In The Synthesis And Hydrolytic Study Of 2-Deoxy-Alpha-D-Glucopyranosyl Phosphate. CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE 1988, 66, 1970-1972.
BERVEN, L. A. ; DOLPHIN, D. H. ; Withers, S. G. A Novel Mechanism Of Glycoside Anomerization. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1988, 110, 4864-4866.
Withers, S. G. ; RUPITZ, K. ; STREET, I. P. 2-Deoxy-2-Fluoro-D-Glycosyl Fluorides - A New Class Of Specific Mechanism-Based Glycosidase Inhibitors. JOURNAL OF BIOLOGICAL CHEMISTRY 1988, 263, 7929-7932.

1987

Withers, S. G. ; STREET, I. P. ; BIRD, P. ; DOLPHIN, D. H. 2-Deoxy-2-Fluoroglucosides - A Novel Class Of Mechanism-Based Glucosidase Inhibitors. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 1987, 109, 7530-7531.
GOLDSMITH, E. J. ; FLETTERICK, R. J. ; Withers, S. G. The 3-Dimensional Structure Of Acarbose Bound To Glycogen-Phosphorylase. JOURNAL OF BIOLOGICAL CHEMISTRY 1987, 262, 1449-1455.

1986

BERVEN, L. A. ; Withers, S. G. A Convenient And High-Yielding Synthesis Of 1,2,3,4,6-Penta-O-Acetyl-Beta-D-[1-H-2]-Glucopyranose. CARBOHYDRATE RESEARCH 1986, 156, 282-285.
Withers, S. G. ; MACLENNAN, D. J. ; STREET, I. P. The Synthesis And Hydrolysis Of A Series Of Deoxyfluoro-D-Glucopyranosyl Phosphates. CARBOHYDRATE RESEARCH 1986, 154, 127-144.
Withers, S. G. ; DOMBROSKI, D. ; BERVEN, L. A. ; KILBURN, D. G. ; MILLER, R. C. ; WARREN, R. A. J. ; GILKES, N. R. Direct H-1-Nmr Determination Of The Stereochemical Course Of Hydrolyzes Catalyzed By Glucanase Components Of The Cellulase Complex. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1986, 139, 487-494.
DAY, A. G. ; Withers, S. G. The Purification And Characterization Of A Beta-Glucosidase From Alcaligenes-Faecalis. BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE 1986, 64, 914-922.
STREET, I. P. ; Withers, S. G. Fluorination Of Protected Mannose Derivatives Using Diethylaminosulfur Trifluoride. CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE 1986, 64, 1400-1403.
Withers, S. G. ; STREET, I. P. ; Rettig, S. J. The Preferred Conformation Of 2-Fluoro-2-Deoxy Beta-D-Mannopyranosyl Fluoride - An X-Ray Crystallographic And Two-Dimensional Proton Nuclear-Magnetic-Resonance Study. CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE 1986, 64, 232-236.

1985

Withers, S. G. Pyridoxal(5')Diphospho(1)-Alpha-D-Glucose - A Potent R-State Inhibitor Of Glycogen-Phosphorylase. JOURNAL OF BIOLOGICAL CHEMISTRY 1985, 260, 841-845.
DAY, A. G. ; Withers, S. G. A Simple Kinetic Test To Distinguish Exo-Glucosidase And Beta-Glucosidase Activities. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 1985, 133, 628-632.
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