|Title||In situ extension as an approach for identifying novel alpha-amylase inhibitors|
|Publication Type||Journal Article|
|Year of Publication||2004|
|Authors||Numao, S, Damager, I, Li, CM, Wrodnigg, TM, Begum, A, Overall, CM, BRAYER, GD, Withers, SG|
|Journal||JOURNAL OF BIOLOGICAL CHEMISTRY|
|Date Published||NOV 12|
A new approach for the discovery and subsequent structural elucidation of oligosaccharide-based inhibitors of alpha-amylases based upon autoglucosylation of known alpha-glucosidase inhibitors is presented. This concept, highly analogous to what is hypothesized to occur with acarbose, is demonstrated with the known alpha-glucosidase inhibitor, D-gluconohydroximino-1,5-lactam. This was transformed from an inhibitor of human pancreatic alpha-amylase with a K-i value of 18 mM to a trisaccharide analogue with a K-i value of 25 muM. The three-dimensional structure of this complex was determined by x-ray crystallography and represents the first such structure determined with this class of inhibitors in any alpha-glycosidase. This approach to the discovery and structural analysis of amylase inhibitors should be generally applicable to other endoglucosidases and readily adaptable to a high throughput format.