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Separation of porphyrin-based photosensitizer isomers by laser-induced fluorescence capillary electrophoresis

TitleSeparation of porphyrin-based photosensitizer isomers by laser-induced fluorescence capillary electrophoresis
Publication TypeJournal Article
Year of Publication2005
AuthorsPeng, XJ, Sternberg, E, Dolphin, D
JournalElectrophoresis
Volume26
Pagination3861-3868
Date PublishedOct
Type of ArticleArticle
ISBN Number0173-0835
Keywordschiral additives, CHLORIN, DERIVATIVES, EFFICACY, ENANTIOMERS, FLUORESCENCE DETECTION, FRACTION, LIGHT, PANCREATIC-CANCER CELLS, PERFORMANCE LIQUID-CHROMATOGRAPHY, pharmacokinetics, PHOTODYNAMIC THERAPY, PHTHALOCYANINES, porphyrins, regioisomers
Abstract

Methods for the separation of photosensitizer isomers, such as benzoporphyrin derivative monoacid, benzoporphyrin ethyl monoacid, 2-[1-hexyloxyethyl]-2-devinylpyropheophorbide-a, diethyleneglycol diester benzoporphyrin derivative, tin ethyl etiopurpurin, and phthalocyanine tetrasulfonate, have been systematically developed by CE. Detection was accomplished by UV absorption at 214 nm or by LIF with excitation at 442/488 nm and emission at 690 nm. The effects of three major experimental parameters of buffer types, organic solvents, and surfactant additives are described. The optimized separation conditions were determined so as to provide satisfactory separation efficiency and analysis time. The methods are shown to be suitable for the separation and determination of porphyrin and phthalocyanines regioisomers, diastereoisomers, and enantiomers.

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