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Separation of free amino acids in human plasma by capillary electrophoresis with laser induced fluorescence: potential for emergency diagnosis of inborn errors of metabolism

TitleSeparation of free amino acids in human plasma by capillary electrophoresis with laser induced fluorescence: potential for emergency diagnosis of inborn errors of metabolism
Publication TypeJournal Article
Year of Publication2001
AuthorsBoulat, O, McLaren, DG, Arriaga, EA, Chen, DDY
JournalJournal of Chromatography B
Volume754
Pagination217-228
Date PublishedApr
Type of ArticleArticle
ISBN Number0378-4347
Keywordsamino acids, CARNITINE, CHROMATOGRAPHY, inborn errors, urine, ZONE ELECTROPHORESIS
Abstract

Free amino acids (AAs) in human plasma are derivatized with 3-(4-carboxybenzoyl)quinoline -2-carboxaldehyde (CBQCA) and analyzed by capillary electrophoresis (CE) with laser induced fluorescence (LIF) detection. The labeling procedure is significantly improved over results reported previously. Derivatization can be completed in 40 min, with concentrations as low as 4x10(-8) M successfully labeled in favourable cases. Twenty-nine AAs (including 2 internal standards) are identified and can be reproducibly separated in 70 min. Migration time RSD values for 23 of these AAs were calculated and found in the range from 0.5 to 4%. The rapid derivatization procedure and the resolution obtained in the separation are sufficient for a semi-quantitative, emergency diagnosis of several inborn errors of metabolism (IEM). Amino acid profiles for both normal donor plasma samples and plasma samples of patients suffering from phenylketonuria, tyrosinemia, maple syrup urinary disease, hyperornithinemia. and citrullinemia are studied. (C) 2001 Elsevier Science B.V. All rights reserved.

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