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Selective focusing of catecholamines and weakly acidic compounds by capillary electrophoresis using a dynamic pH junction

TitleSelective focusing of catecholamines and weakly acidic compounds by capillary electrophoresis using a dynamic pH junction
Publication TypeJournal Article
Year of Publication2000
AuthorsBritz-McKibbin, P, Chen, DDY
JournalAnalytical Chemistry
Volume72
Pagination1242-1252
Date PublishedMar
Type of ArticleArticle
ISBN Number0003-2700
KeywordsDNA-ADDUCTS, ELECTROCHEMICAL DETECTION, INJECTION, MICELLAR ELECTROKINETIC CHROMATOGRAPHY, MIGRATING MICELLES, NEUTRAL ANALYTES, REVERSE, SAMPLE SELF-STACKING, SEPARATION, VOLUME, ZONE-ELECTROPHORESIS
Abstract

A systematic study of selective analyte focusing in a multisection electrolyte system by capillary electrophoresis (CE) is presented. It was found that a dynamic pH junction between sample and background electrolyte zones can be used to focus zwitterionic catecholamines and weakly acidic compounds without the use of special ampholytes. Differences in pH and concentration of complexing agents, such as berate, in the sample and background electrolyte zones were determined to cause focusing through changes in the local velocity of the analyte in two different segments of the capillary. Velocity-differerence induced focusing (V-DIF) of analytes using a dynamic pH junction allowed the injection of large sample volumes and significantly improved the concentration sensitivity of CE. Under optimized conditions, the limit of detection for epinephrine was determined to be about 4 x 10(-8) M (the original sample) with conventional UV absorbance detection. Moreover, separation efficiencies greater than a million theoretical plates can be achieved by focusing such large sample volumes into narrow zones. Multisection electrolyte systems, which lead to the formation of a dynamic pH junction, can be tuned toward improving the concentration sensitivity of specific analytes their chemical properties are known.

URL<Go to ISI>://000085975200021