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Selection and characterization of DNAzymes with synthetically appended functionalities: A case of a synthetic RNaseA mimic

TitleSelection and characterization of DNAzymes with synthetically appended functionalities: A case of a synthetic RNaseA mimic
Publication TypeJournal Article
Year of Publication2004
AuthorsTing, R, Lermer, L, Thomas, J, Roupioz, Y, Perrin, DM
JournalPure and Applied Chemistry
Volume76
Pagination1571-1577
Date PublishedJul-Aug
Type of ArticleProceedings Paper
ISBN Number0033-4545
KeywordsCATALYTIC, CLEAVING DNA ENZYME, DIVALENT METAL-ION, IMIDAZOLYL FUNCTIONALITIES, IN-VITRO SELECTION, MONOVALENT CATIONS, NUCLEIC-ACIDS, NUCLEOSIDE 5’-TRIPHOSPHATES, REPERTOIRE, SEQUENCE-SPECIFIC CLEAVAGE, SITE-SPECIFIC CLEAVAGE
Abstract

We have been interested in merging synthetic nucleotide chemistry with combinatorial selection of DNAzymes to deliver a more complete (and complex) chemical complement to the catalytic repertoire of nucleic acids. Thus we ask, what do modified dNTPs really bring to nucleic acids in terms of an increased repertoire? In asking this question, we have looked first at conditions, and more recently for reaction classes where nucleic acids are found to be catalytically inefficient, deficient, or at least to date, seemingly incapable of certain functions. A case of this is M2+-independent ribophosphodiester hydrolysis at physiological pH and low ionic strength where nucleic acids exhibit especially low rate constants for self-cleavage and seem to be incapable of turnover.

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