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Quantitative analysis of propofol in whole blood using capillary electrophoresis

TitleQuantitative analysis of propofol in whole blood using capillary electrophoresis
Publication TypeJournal Article
Year of Publication2009
AuthorsHui, Y, Raedschelders, K, Zhang, H, Ansley, DM, Chen, DDY
JournalJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume877
Pagination703-709
Date PublishedMar
Type of ArticleArticle
ISBN Number1570-0232
KeywordsAortocoronary bypass, BIOPHARMACEUTICAL INDUSTRY, BREATH, capillary electrophoresis, Cardiopulmonary bypass, CHROMATOGRAPHY-MASS-SPECTROMETRY, grafting, HUMAN, Method development, PLASMA, POPULATION, Propofol, Whole blood
Abstract

We have developed a straightforward capillary electrophoresis method capable of quantifying clinically relevant propofol concentrations in whole blood from patients undergoing aortocoronary bypass grafting with cardiopulmonary bypass. The method utilizes 400 mu L of whole blood and is capable of detecting propofol in the ng/mL range. Factors affecting reproducibility and reliability of analytical results for clinically relevant samples are discussed. The method was used to evaluate propofol concentrations in blood samples from 30 patients. The distribution in the whole blood concentration achieved in patients advocates the need for target-achieved monitoring techniques. (C) 2009 Elsevier B.V. All rights reserved.

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