|Title||Potential new inorganic antitumour agents from combining the anticancer traditional Chinese medicine (TCM) liriodenine with metal ions, and DNA binding studies|
|Publication Type||Journal Article|
|Year of Publication||2009|
|Authors||Chen, ZF, Liu, YC, Liu, LM, Wang, HS, Qin, SH, Wang, BL, Bian, HD, Yang, B, Fun, HK, Liu, HG, Liang, H, Orvig, C|
|Type of Article||Article|
|Keywords||AFFINITY, ALKALOIDS, CISPLATIN, CYTOTOXIC ACTIVITY, DRUGS, FLUORESCENCE, IN-VITRO, PLATINUM(II) COMPLEXES, RUTHENIUM(II) COMPLEXES, TOPOISOMERASE-II|
Liriodenine (L), an active component of the anticancer traditional Chinese medicine (TCM), was isolated from Zanthoxylum nitidum. Its reactions with Pt(II) and Ru(II) afforded three metal complexes: cis-[PtCl2(L)] (1), cis-[PtCl2(L)(DMSO)] (2), and cis-[RuCl2(L)(DMSO)(2)]center dot 1.5H(2)O (3), the crystal structures of L, 2 and 3 were determined by single-crystal X-ray diffraction methods. These complexes were fully characterized by elemental analysis, IR spectrophotometry, H-1 and C-13 NMR spectroscopies, and ES mass spectrometry. The in vitro cytotoxicity of L and complexes 1-3 against 11 human tumour cell lines was assayed. The metal-based compounds exhibit enhanced cytotoxicity vs. free L, suggesting that these compounds display synergy in the combination of metal ions and liriodenine. The binding properties of L and its complexes 1-3 to ct-DNA were investigated through UV-vis, fluorescence, CD spectra, viscosity and agarose gels electrophoretic measurements.
|URL||<Go to ISI>://000261807400006|