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Novel natural products from soil DNA libraries in a streptomycete host

TitleNovel natural products from soil DNA libraries in a streptomycete host
Publication TypeJournal Article
Year of Publication2000
AuthorsWang, GYS, Graziani, E, Waters, B, Pan, WB, Li, X, McDermott, J, Meurer, G, Saxena, G, Andersen, RJ, Davies, J
JournalOrganic Letters
Volume2
Pagination2401-2404
Date PublishedAug
Type of ArticleArticle
ISBN Number1523-7060
Abstract

[GRAPHICS] As a route to accessing the potential chemical diversity of uncultivable microbes from the soil, combinatorial biosynthetic libraries were constructed by cloning large fragments of DNA isolated from soil into a Streptomyces lividans host. Four novel compounds, terragines A (1), B (2), C (3), and D (4), were isolated from recombinant 436-s4-5b1, and another novel compound, terragine E (5), was isolated from 446-s3-102g1. The structures were determined by a combination of spectroscopic techniques, primarily 2D NMR.

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