|Title||Novel natural products from soil DNA libraries in a streptomycete host|
|Publication Type||Journal Article|
|Year of Publication||2000|
|Authors||Wang, GYS, Graziani, E, Waters, B, Pan, WB, Li, X, McDermott, J, Meurer, G, Saxena, G, Andersen, RJ, Davies, J|
|Type of Article||Article|
[GRAPHICS] As a route to accessing the potential chemical diversity of uncultivable microbes from the soil, combinatorial biosynthetic libraries were constructed by cloning large fragments of DNA isolated from soil into a Streptomyces lividans host. Four novel compounds, terragines A (1), B (2), C (3), and D (4), were isolated from recombinant 436-s4-5b1, and another novel compound, terragine E (5), was isolated from 446-s3-102g1. The structures were determined by a combination of spectroscopic techniques, primarily 2D NMR.
|URL||<Go to ISI>://000088605200003|