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Novel electrophoresis mechanism based on synchronous alternating drag perturbation

TitleNovel electrophoresis mechanism based on synchronous alternating drag perturbation
Publication TypeJournal Article
Year of Publication2005
AuthorsMarziall, A, Pel, J, Bizzotto, D, Whitehead, LA
JournalElectrophoresis
Volume26
Pagination82-90
Date PublishedJan
Type of ArticleArticle
ISBN Number0173-0835
KeywordsDNA, drag alteration, ELECTROPHORESIS, ENTROPIC RATCHETS, focusing, mobility, multidimensional
Abstract

We present a novel means of transporting molecules in solution by applying a zero-time-average alternating motive force to the molecules, and perturbing the molecular drag coefficient synchronously with the applied force, thus causing a net drift in a direction determined by the phase of the alternating drag perturbation relative to the alternating force. We apply an electrophoretic form of the method to transport and concentrate DNA in a gel, such that all molecules migrate on average away from the nearest electrode and toward a central region. Since an electrode does not occupy this central region, this method presents the possibility of transporting and focusing DNA and other charged molecules in regions free from electrodes and the associated electrochemistry.

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