Research & Faculty

Default Header Image

Modification of silica with a covalently attached antigen for use in immunosorbent assays

TitleModification of silica with a covalently attached antigen for use in immunosorbent assays
Publication TypeBook Chapter
Year of Publication1995
AuthorsMelzak, KA, Brooks, DE
EditorHorbett, TA, Brash, JL
Book TitleProteins at Interfaces Ii - Fundamentals and Applications
Series TitleAcs Symposium Series
Volume602
Pagination420-433
PublisherAmer Chemical Soc
CityWashington
ISBN Number0097-61560-8412-3304-7
KeywordsTEMPERATURE, XPS
Abstract

This paper describes the preparation and characterization of a silica surface with a covalently coupled antigen, and gives an example of the use of the modified silica in immunosorbent assays. Silica beads and slides were cleaned with chromic acid and modified with 3-aminopropyltriethoxysilane. The carboxyl groups of a cyclic hexapeptide antigen, ferrichrome A, were coupled to the amine-modified silica using 1-ethyl,3,3-dimethylaminopropyl cabodiimide to promote formation of a peptide bond. Modified slides and beads were characterized with a combination of X-ray photoelectron spectroscopy and particle electrophoresis measurements, with the amount of ferrichrome A adsorbed of coupled to the beads being determined by solution depletion measurements.

URL<Go to ISI>://A1995BE38N00030