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A Method for Determining the Actual Rate of Orientation Switching of DNA Self-Assembled Monolayers Using Optical and Electrochemical Frequency Response Analysis

TitleA Method for Determining the Actual Rate of Orientation Switching of DNA Self-Assembled Monolayers Using Optical and Electrochemical Frequency Response Analysis
Publication TypeJournal Article
Year of Publication2015
AuthorsCasanova-Moreno, J, Bizzotto, D
JournalAnal. Chem.
Volume87
Pagination2255–2263
Abstract

Electrostatic control of the orientation of fluorophore-labeled DNA strands immobilized on an electrode surface is an effective bioanal. tool. Modulation techniques and later time-resolved measurements were used to evaluate the kinetics of the switching between lying and standing DNA conformations. These measurements, however, are the result of a convolution between the DNA "switching" response time and the other frequency limited responses in the measurement. A method for analyzing the response of a potential driven DNA sensor is presented by calcg. the potential effectively dropped across the electrode interface (using electrochem. impedance spectroscopy) as opposed to the potential applied to the electrochem. cell. This effectively deconvolutes the effect of the charging time on the obsd. frequency response. The cor. response shows that DNA is able to switch conformation faster than previously reported using modulation techniques. This approach will ensure accurate measurements independent of the electrochem. system, removing the uncertainty in the anal. of the switching response, enabling comparison between samples and measurement systems. [on SciFinder(R)]

URLhttp://pubs.acs.org/doi/abs/10.1021/ac503919a
DOI10.1021/ac503919a