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A human protein atlas for normal and cancer tissues based on antibody proteomics

TitleA human protein atlas for normal and cancer tissues based on antibody proteomics
Publication TypeJournal Article
Year of Publication2005
AuthorsUhlen, M, Bjorling, E, Agaton, C, Szigyarto, CA, Amini, B, Andersen, E, Andersson, AC, Angelidou, P, Asplund, A, Asplund, C, Berglund, L, Bergstrom, K, Brumer, H, Cerjan, D, Ekstrom, M, Elobeid, A, Eriksson, C, Fagerberg, L, Falk, R, Fall, J, Forsberg, M, Bjorklund, MG, Gumbel, K, Halimi, A, Hallin, I, Hamsten, C, Hansson, M, Hedhammar, M, Hercules, G, Kampf, C, Larsson, K, Linskog, M, Lodewyckx, W, Lund, J, Lundeberg, J, Magnusson, K, Malm, E, Nilsson, P, Odling, J, Oksvold, P, Olsson, I, Oster, E, Ottosson, J, Paavilainen, L, Persson, A, Rimini, R, Rockberg, J, Runeson, M, Sivertsson, A, Skollermo, A, Steen, J, Stenvall, M, Sterky, F, Stromberg, S, Sundberg, M, Tegel, H, Tourle, S, Wahlund, E, Walden, A, Wan, JH, Wernerus, H, Westberg, J, Wester, K, Wrethagen, U, Xu, LL, Hober, S, Ponten, F
JournalMOLECULAR & CELLULAR PROTEOMICS
Volume4
Pagination1920-1932
Date PublishedDEC
Type of ArticleArticle
ISSN1535-9476
Abstract

Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, similar to 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.

DOI10.1074/mcp.M500279-MCP200