|Title||An ent-kaurene that inhibits mitotic chromosome movement and binds the kinetochore protein Ran-binding protein 2|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Rundle, NT, Nelson, J, Flory, MR, Joseph, J, Th’ng, J, Aebersold, R, Dasso, M, Andersen, RJ, Roberge, M|
|Journal||Acs Chemical Biology|
Using a chemical genetics screen, we have identified ent-15-oxokaurenoic acid (EKA) as a chemical that causes prolonged mitotic arrest at a stage resembling prometaphase. EKA inhibits the association of the mitotic motor protein centromeric protein E with kinetochores and inhibits chromosome movement. Unlike most antimitotic agents, EKA does not inhibit the polymerization or depolymerization of tubulin. To identify EKA-interacting proteins, we used a cell-permeable biotinylated form that retains biological activity to isolate binding proteins from living cells. Mass spectrometric analysis identified six EKA-binding proteins, including Ran-binding protein 2, a kinetochore protein whose depletion by small interfering RNA causes a similar mitotic arrest phenotype.
|URL||<Go to ISI>://000243894000018|