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A divalent metal-dependent self-cleaving DNAzyme with a tyrosine side chain

TitleA divalent metal-dependent self-cleaving DNAzyme with a tyrosine side chain
Publication TypeJournal Article
Year of Publication2011
AuthorsLam, CH, Hipolito, CJ, Hollenstein, M, Perrin, DM
JournalOrg. Biomol. Chem.
Volume9
Pagination6949-6954
Abstract

The enzymatic incorporation of a phenol-modified 2[prime or minute]-deoxyuridine triphosphate gave rise to a modified DNA library that was subsequently used in an in vitro selection for ribophosphodiester-cleaving DNAzymes in the presence of divalent zinc and magnesium cations. After 11 rounds of selection{,} cloning and sequencing resulted in 14 distinct sequences{,} the most active of which was Dz11-17PheO. Dz11-17PheO self-cleaved an embedded ribocytidine with an observed rate constant of 0.20 +/- 0.02 min-1 in the presence of 10 mM Mg2+ and 1 mM Zn2+ at room temperature. The activity was inhibited at low concentrations of Hg2+ cations and somewhat higher concentrations of Eu3+ cations.

URLhttp://dx.doi.org/10.1039/C1OB05359K
DOI10.1039/C1OB05359K