|Title||Dissection of nucleophilic and acid-base catalysis in glycosidases|
|Publication Type||Journal Article|
|Year of Publication||2001|
|Authors||Zechel, DL, Withers, SG|
|Journal||CURRENT OPINION IN CHEMICAL BIOLOGY|
A startling array of added anions have been observed to function as replacement catalytic nucleophiles in mutant glycosidases, including formate, azide, fluoride and other halides. Likewise, the mechanism of acid-base catalysis is somewhat plastic. The carboxylic acids can be substituted by a sulfenic acid or by ascorbate, and the effective acid strength enhanced by the introduction of strong hydrogen bonds, These studies provide an interesting bridge between enzymes and models thereof.