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Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides

TitleCrystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides
Publication TypeJournal Article
Year of Publication2003
AuthorsJohansson, P, Denman, S, Brumer, H, Kallas, AM, Henriksson, H, Bergfors, T, Teeri, TT, Jones, TA
JournalACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
Volume59
Pagination535-537
Date PublishedMAR
Type of ArticleArticle
ISSN0907-4449
Abstract

{Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 Angstrom resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6

DOI10.1107/S090744490202348X