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Continuous electrophoretic purification of individual analytes from multicomponent mixtures

TitleContinuous electrophoretic purification of individual analytes from multicomponent mixtures
Publication TypeJournal Article
Year of Publication2004
AuthorsMcLaren, DG, Chen, DDY
JournalAnalytical Chemistry
Volume76
Pagination2298-2305
Date PublishedApr
Type of ArticleArticle
ISBN Number0003-2700
KeywordsCAPILLARY-ZONE-ELECTROPHORESIS, CHIRAL SEPARATIONS, CHROMATOGRAPHY, ENANTIOMERS, FRACTION COLLECTOR, FREE-FLOW ELECTROPHORESIS, ISOELECTRIC-FOCUSING SEPARATION, MASS-SPECTROMETRY, MEMBRANE, PREPARATIVE-SCALE
Abstract

Individual analytes can be isolated from multicomponent mixtures and collected in the outlet vial by carrying out electrophoretic purification through a capillary column. Desired analytes are allowed to migrate continuously through the column under the electric field while undesired analytes are confined to the inlet vial by application of a hydrodynamic counter pressure. Using pressure ramping and buffer replenishment techniques, 18% of the total amount present in a bulk sample can be purified when the resolution to the adjacent peak is similar to3. With a higher resolution, the yield could be further improved. Additionally, by periodically introducing fresh buffer into the sample, changes in pH and conductivity can be mediated, allowing higher purity (99.5%) to be preserved in the collected fractions. With an additional reversed cycle of flow counterbalanced capillary electrophoresis, any individual component in a sample mixture can be purified providing it can be separated in an electrophoresis system.

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