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Capillary Electrophoresis Mass Spectrometry for the Characterization of O-Acetylated N-Glycans from Fish Serum

TitleCapillary Electrophoresis Mass Spectrometry for the Characterization of O-Acetylated N-Glycans from Fish Serum
Publication TypeJournal Article
Year of Publication2012
AuthorsJayo, RG, Li, J, Chen, DDY
JournalANALYTICAL CHEMISTRY
Volume84
Pagination8756-8762
Date PublishedOCT 16
ISSN0003-2700
Abstract

O-Acetylated N-glycans from fish serum of Atlantic salmon (Salmo solar) are characterized by capillary electrophoresis (CE) in conjunction with both laser-induced fluorescence (LIF) and mass spectrometry (MS) detection methods. Glycans derivatized with negatively charged fluorescent label 8-aminopyrene-1,2,6-trisulfonate (APTS) were separated to obtain a CE-LIF profile of the complex glycan mixture, and the profile concurs with that obtained by using electrospray mass spectrometry. The identity of the APTS-labeled glycans was confirmed by CE-MS. The same glycans can be identified also in their native state by CE MS without derivatization. The structural variations of O-acetylated sialic acid isomers in fish serum glycans are investigated by CE-MS/MS. Selected ion monitoring provided useful structural information of the underivatized glycans from fragmentation spectra. New complex fish serum glycans that are not reported previously were observed and characterized. These methods may be useful not only for the characterization of acetylation of complex glycans but also to study other types of glycan modifications, as well. as to allow determination of overall glycan composition in glycoproteins.

DOI10.1021/ac301889k