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Capillary electrophoresis-mass spectrometry for analysis of complex samples

TitleCapillary electrophoresis-mass spectrometry for analysis of complex samples
Publication TypeJournal Article
Year of Publication2012
AuthorsZhao, SSherry, Zhong, X, Tie, C, Chen, DDY
JournalPROTEOMICS
Volume12
Pagination2991-3012
Date PublishedOCT
ISSN1615-9853
Abstract

CE features superior separation efficiency, small solvent consumption, as well as the ability to analyze most biomolecules with an open tube fused-silica column. When coupled with MS, the separation power of CE is enhanced by adding another separation dimension based on mass-to-charge ratios. CE-MS reduces the dependence on CE separation so that faster analysis can be achieved. It also yields higher sensitivity as well as the capability for analyte identification and structural elucidation. The use of CE-MS for biomolecule analysis has increased significantly in the last 5 years. New methods are being developed for large molecules, while analyses of smaller molecules are moving toward the study in more complex tissues and other matrices. In this article, the applications of CE-ESI-MS for complex samples in 20072011 are reviewed. The applications are categorized according to the types of analytes studied, including the analysis for proteins and peptides, carbohydrates, and small biomolecules. Sample preparation methods, coatings for capillary inner wall, online processing strategies, and other aspects are also reviewed in each category.

DOI10.1002/pmic.201200221