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Behavior of interacting species in vacancy affinity capillary electrophoresis described by mass balance equation

TitleBehavior of interacting species in vacancy affinity capillary electrophoresis described by mass balance equation
Publication TypeJournal Article
Year of Publication2008
AuthorsSun, Y, Fang, N, Chen, DDY
JournalElectrophoresis
Volume29
Pagination3333-3341
Date PublishedAug
Type of ArticleArticle
ISBN Number0173-0835
Keywordsaffinity capillary electrophoresis, binding constant, COMPUTER-SIMULATION, CONSTANTS, DRUG-PROTEIN-BINDING, equation, EXPERIMENTAL VALIDATION, FRONTAL ANALYSIS, HUMAN-SERUM-ALBUMIN, HUMMEL-DREYER, mass balance, method, PERFORMANCE LIQUID-CHROMATOGRAPHY, vacancy affinity capillary electrophoresis, WALL ADSORPTION, ZONE-ELECTROPHORESIS
Abstract

Vacancy ACE (VACE) is one of the ACE methods, and has been used to study binding interactions between different biomolecules. Thermodynamic binding constants can be estimated with nonlinear regression methods. With a highly efficient computer simulation program (SimDCCE), it is possible to demonstrate the detailed behaviors of each species during the interaction process under different conditions. In this work, thirteen scenarios in four different combinations of migration orders of the free protein, free drug, and complex formed are studied. The detailed interaction process between protein and ligand is discussed and illustrated based on the mass balance equation, also called mass transfer equation. By properly setting the parameters in the simulation model, the influence of different factors during the interaction process can be well understood.

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