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Artificial nucleases

TitleArtificial nucleases
Publication TypeJournal Article
Year of Publication2001
AuthorsChen, CHB, Milne, L, Landgraf, R, Perrin, DM, Sigman, DS
JournalChembiochem
Volume2
Pagination735-740
Date PublishedOct
Type of ArticleArticle
ISBN Number1439-4227
Keywords1, 10-PHENANTHROLINE-COPPER ION, chemical, COLI RNA-POLYMERASE, COMPLEX, copper, DNA, DNA cleavage, DNA recognition, ESCHERICHIA-COLI, NUCLEASE, nucleic acids, OLIGONUCLEOTIDES, PROTEIN, SITE-SPECIFIC NUCLEASE, TRANSCRIPTION INITIATION
Abstract

The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids wit proteins and oligonucleotides In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(I) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline - oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(I).

URL<Go to ISI>://000171410000003