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Analysis of amphetamine, methamphetamine and methylenedioxy-methamphetamine by micellar capillary electrophoresis using cation-selective exhaustive injection

TitleAnalysis of amphetamine, methamphetamine and methylenedioxy-methamphetamine by micellar capillary electrophoresis using cation-selective exhaustive injection
Publication TypeJournal Article
Year of Publication2006
AuthorsMeng, PJ, Fang, N, Wang, M, Liu, HW, Chen, DDY
JournalElectrophoresis
Volume27
Pagination3210-3217
Date PublishedAug
Type of ArticleArticle
ISBN Number0173-0835
Keywordsamphetamine, AMPLIFIED SAMPLE STACKING, analysis, cation-selective exhaustive injection, CHROMATOGRAPHY, CHROMATOGRAPHY-MASS SPECTROMETRY, ELECTROKINETIC, FLUORESCENCE DETECTION, illicit drug, LIQUID-CHROMATOGRAPHY, methamphetamine, methylenedioxy-methamphetamine, NEUTRAL ANALYTES, ONLINE CONCENTRATION, REVERSE MIGRATING MICELLES, SOLID-PHASE MICROEXTRACTION, ZONE-ELECTROPHORESIS
Abstract

Cation-selective exhaustive injection (CSEI) is used as an on-line concentration method for the high-sensitivity analysis of illicit amphetamines using CE. Optimum conditions for the determination of amphetamine, methamphetamine and methylenedioxy-methamphetamine were investigated. Sodium dodecyl sulfate (25 mM) in 100 mM phosphate buffer (pH 2.9) with 20% methanol as organic additive was used as the background electrolyte for CE separation. The LOD, based on an S/N of 3:1, was about 0.01 mu g/mL using normal capillary micellar electrokinetic chromatography, while by using CSEI in combination with micellar sweeping the sensitivity increased up to 1000-fold with the LOD lower than 50 pg/mL. The reproducibility of CSEI combined with micellar sweeping for analyzing amphetamines was satisfactory (relative standard deviation around 10% by using area ratios against an internal standard). This method is highly sensitive and can be used to analyze trace amount amphetamines in human hair.

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